All Issue

2020 Vol.38, Issue 1 Preview Page

Research Article

Resistance of Commercial Radish Cultivars to Isolates of Fusarium oxysporum f. sp. raphani

시들음병균 균주들에 대한 무 품종들의 저항성

Soo Min Lee1
,
Ji Hyun Lee1
,
Kyoung Soo Jang1
,
Yong Ho Choi1
,
Hun Kim1
,
Gyung Ja Choi1*
이 수민1
,
이 지현1
,
장 경수1
,
최 용호1
,
김 헌1
,
최 경자1*
1Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology
1한국화학연구원 친환경신물질연구센터
* Corresponding Author
28 February 2020. pp. 97-106
PDF XML Citation
Abstract

Fusarium wilt of radish caused by Fusarium oxysporum f. sp. raphani (FOR) is one of the most severe diseases in radish cultivation. To define resistance characteristics of radish cultivars to FOR, we tested virulence of 8 FOR isolates in a susceptible cultivar of radish, ‘Backchun’. Area under disease progress curve (AUDPC) of Fusarium wilt on the cultivar caused by FOR 60A, 59A, 57A, 147A, KR1, NW1, HN and JHW was 6.7, 14, 16, 27, 27, 30, 35 and 35, respectively. Thus, virulence of FOR HN and JHW was the strongest, followed by FOR NW1, KR1, 147A, 57A, 59A and 60A isolates. In addition, we investigated development of Fusarium wilt on 60 commercial radish cultivars caused by FOR KR1, a medium virulent isolate of them. From the results, 25 radish cultivars were selected for further study. When the cultivars were inoculated with the seven FOR isolates except for KR1, mean disease index of the cultivars by FOR 60A, 59A, 57A, 147A, NW1, HN, and JHW isolates were 0.5, 0.7, 1.2, 2.3, 2.8, 3.7 and 3.9, respectively. And the number of resistant radish cultivars to FOR 60A, 59A, 57A, 147A, NW1, HN, and JHW were 21, 20, 13, 7, 2, 1 and 1, respectively. Furthermore, development of Fusarium wilt in each cultivar was positively correlated with the virulence of FOR isolates. Taken together, these results suggest that resistance of radish to FOR isolates is likely affected by the virulence, but not resulted from race differentiation.

Keywords
breeding
disease resistance
fusarium wilt
race
virulence

무 시들음병은 Fusarium oxysporum f. sp. raphani (FOR)에 의해 발생하며, 무 재배지에서 심각한 피해를 주고 있는 병들 중 하나이다. 무 품종들의 시들음병 저항성 특징을 조사하기 위하여, 감수성 품종인 ‘백춘’에 FOR 8개 균주를 접종하고 시들음병 발생을 조사하였다. FOR 60A, 59A, 57A, 147A, KR1, NW1, HN, JHW에 의한 ‘백춘’에서의 병진전곡선하면적(AUDPC)은 각각 6.7, 14, 16, 27, 27, 30, 35, 35였다. 즉, FOR 균주들의 병원력은 HN와 JHW 균주가 가장 강하였고, 그 다음은 NW1, 147A, 57A, 59A, 60A 순이었다. 그리고 이들 균주 중 중간 정도의 병원력을 나타내는 FOR KR1 균주를 사용하여 시판 무 품종 60개의 시들음병 발생을 조사하였다. 이로부터 시들음병 저항성 특성 실험을 위하여 25개 품종을 선발하였다. FOR KR1 균주를 제외한 7개 균주에 의한 선발한 25개 품종의 시들음병 발생을 조사한 결과, 무 시들음병균 FOR 60A, 59A, 57A, 147A, NW1, HN, JHW에 의한 평균 발병도는 각각 0.5, 0.7, 1.2, 2.3, 2.8, 3.7, 3.9였으며, 저항성 품종의 수는 각각 21개, 20개, 13개, 7개, 2개, 1개 및 1개 이었다. 그리고 각 품종에서의 시들음병 발생은 FOR 균주들의 병원력에 비례하였다. 이상의 결과로 무의 시들음병 저항성은 F. oxysporum f. sp. raphani 균주들의 병원력 정도에 따라 영향을 받는 것이며, 병원균의 레이스(race) 분화 때문이 아닌 것으로 생각되었다.

키워드
육종
병 저항성
시들음병
레이스
병원력
References
1
Abebe AM, Wai KPP, Siddique MI, Mo H-S, Yoo HJ, Jegal Y, Byeon S-E, Jang K-S, Jeon S-G, et al. (2016) Evaluation of Phytophthora root rot- and bacterial wilt-resistant inbred lines and their crosses for use as rootstocks in pepper (Capsicum annuum L.). Hortic Environ Biotechnol 57:598-605. doi:10.1007/s13580-016-0050-8
10.1007/s13580-016-0050-8
2
Armstrong GM, Armstrong JK (1952) Physiological races of Fusaria causing wilts of Cruciferae. Phytopathology 42:255-257
3
Armstrong GM, Armstrong JK (1966) Races of Fusarium oxysporum f. conglutinate; race 4, new race; and a new host for race 1; Lychnis chalcedonica. Phytopathology 56:525-530
4
Baik S-Y, Jang KS, Choi YH, Kim J-C, Choi GJ (2011) Resistance degree of radish cultivars to Fusarium oxysporum f. sp. raphani according to several conditions. Korean J Hortic Sci Technol 29:48-52
5
Baik S-Y, Kim J-C, Jang KS, Choi YH, Choi GJ (2010) Development of effective screening method and evaluation of radish cultivars for resistance to Fusarium oxysporum f. sp. raphani. Res Plant Dis 16:148-152. doi:10.5423/RPD.2010.16.2.148
10.5423/RPD.2010.16.2.148
6
Baker KF (1948) Fusarium wilt of garden stock (Mathiola incana) Phytopathology 32:1031-1033
7
Bosland PW, Williams PH (1987) An evaluation of Fusarium oxysporum from crucifers based on pathogenicity, isozyme polymorphism, vegetative compatibility, and geographic origin. Can J Bot 65:2067-2073. doi:10.1139/b87-282
10.1139/b87-282
8
Bosland PW, Williams PH, Morrison RH (1988) Influence of soil temperature on the expression of yellows and wilt of crucifers by Fusarium oxysporum. Plant Dis 72:777-780. doi:10.1094/PD-72-0777
10.1094/PD-72-0777
9
du Toit LJ, Pelter GQ (2003) Wilt of radish caused by Fusarium oxysporum f. sp. raphani in Washington State. Online. Plant Health Progress. doi:10.1094/PHP-2003-0616-01-HN
10.1094/PHP-2003-0616-01-HN
10
Foster JM, Hausbeck MK (2010) Resistance of pepper to Phytophthora crown, root, and fruit rot is affected by isolate virulence. Plant Dis 94:24-30. doi:10.1094/PDIS-94-1-0024
10.1094/PDIS-94-1-002430754398
11
Hibberd AM, Bassett MJ, Stall RE (1987) Allelism tests of three dominant genes for hypersensitive resistance to bacterial spot of pepper. Phytopathology 77:1304-1307. doi:10.1094/Phyto-77-1304
10.1094/Phyto-77-1304
12
Hirai M (2006) Genetic analysis of clubroot resistance in Brassica crops. Breed Sci 56:223-229. doi:10.1270/jsbbs.56.223
10.1270/jsbbs.56.223
13
James RV, Williams PH (1980) Clubroot resistance and linkage in Brassica campestris. Phytopathology 70:776-779. doi:10.1094/Phyto-70-776
10.1094/Phyto-70-776
14
Jayaraman J, Segonzac C, Cho H, Jung G, Sohn KH (2016) Effector-assisted breeding for bacterial wilt resistance in horticultural crops. Hortic Environ Biotechnol 57:415-423. doi:10.1007/s13580-016-0191-9
10.1007/s13580-016-0191-9
15
Jo EJ, Jang KS, Choi YH, Ahn KG, Choi GJ (2016) Resistance of cabbage plants to isolates of Plasmodiophora brassicae. Korean J Hortic Sci Technol 34:442-452. doi:10.12972/kjhst.20160045
10.12972/kjhst.20160045
16
Jo S-J, Shim S-A, Jang KS, Choi YH, Kim J-C, Choi GJ (2014) Resistance of chili pepper cultivars to isolates of Phytophthora capsici. Hortic Sci Technol 32:66-76 (In Korean). doi:10.7235/hort.2014.13079
10.7235/hort.2014.13079
17
Kim H, Hwang S-M, Lee JH, Oh M, Han JW, Choi GJ (2017) Specific PCR detection of Fusarium oxysporum f. sp. raphani: A causal agent of Fusarium wilt on radish plants. Lett Appl Microbiol 65:133-140. doi:10.1111/lam.12761
10.1111/lam.1276128585248
18
Kendrick JB, Snyder WC (1942) Fusarium wilt of radish. Phytopathology 42:1031-1033
19
Kim H, Jo EJ, Choi YH, Jang KS, Choi GJ (2016) Pathotype classification of Plasmodiophora brassicae isolates using clubroot-resistant cultivar of Chinese cabbage. Plant Pathol J 32:423-430. doi:10.5423/PPJ.OA.04.2016.0081
10.5423/PPJ.OA.04.2016.008127721692PMC5051561
20
Kim JH, Bhandari SR, Chae SY, Cho MC, Lee JG (2019) Application of maximum quantum yield, a parameter of chlorophyll fluorescence, for early determination of bacterial wilt in tomato seedlings. Hortic Environ Biotechnol 60:821-829. doi:10.1007/s13580-019-00182-0
10.1007/s13580-019-00182-0
21
Kuginuki Y, Yoshikawa H, Hirai M (1999) Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot resistance cultivars of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Eur J Plant Pathol 105:327-332. doi:10.1023/A:1008705413127
10.1023/A:1008705413127
22
Lee JH, Jang KS, Choi YH, Kim H, Choi GJ (2016) Development of an efficient mass-screening method for testing the resistance of radish to Fusarium wilt. Res Plant Dis 22:152-157. doi:10.5423/RPD.2016.22.3.152
10.5423/RPD.2016.22.3.152
23
Lee JH, Lee JD, Oh D-G (2018) Resistance of pepper cultivars to Ralstonia solanacearum isolates from major cultivated Areas of chilli peppers in Korea. Hortic Sci Technol 36:569-576. doi:10.12972/kjhst.20180057
10.12972/kjhst.20180057
24
Moon YG, Kim WG, Cho WD, Sung JM (2001) Occurrence of Fusarium wilt on cruciferous vegetable crops and pathogenic differentiation of causal fungus. Res Plant Dis 7:93-101
25
Pound GS, Fowler DL (1953) Fusarium wilt of radish in Wisconsin. Phytopathology 43:277-280
26
Ramirez-Villupadua J, Endo RM, Bosland P, Williams PH (1985) A new race of Fusarium oxysporum f. sp. conglutinans that attacks cabbage type A resistance. Plant Dis 69:612-613. doi:10.1094/PD-69-612
10.1094/PD-69-612
27
Risser G, Banihashemi Z, Davis DW (1976) A proposed nomenclature of Fusarium oxysporum f. sp. melonis races and resistance genes in Cucumis melo. Phytopathology 66:1105-1106. doi:10.1094/Phyto-66-1105
10.1094/Phyto-66-1105
28
Snyder W (1949) Cross inoculations with the vascular fusaria of stock cabbage and radish. Phytopathology 39:863
29
Snyder WC, Hansen HN (1940) The species concept in Fusarium. Amer Jour Bot 27:64-67. doi:10.1002/j.1537-2197.1940.tb14217.x
10.1002/j.1537-2197.1940.tb14217.x
30
van Peer R, Xu T, Rattink H, Schippers B (1988) Biological control of carnation wilt caused by Fusarium oxysporum f. sp. dianthi in hydroponic system. ISOSC Proc 361-373.
31
Yang SS, Sung NK, Choi DI, Kim CH (1989) Pathogenic variation of Phytophthora capsici Leonian on red-pepper in Korea. Korean J Plant Pathol 5:370-376
32
Yoshikawa H (1983) Breeding for clubroot resistance of crucifer crops in Japan. Jpn Agr Quart 17:6-11
33
Yoshikawa H (1993) Studies on breeding of cluroot resistance in cole (cruciferae) crop. Bull Natl Res Inst Veg Ornam Plants Tea Japan, Ser A, No 7:1-165
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :1
  • Pages :97-106
  • Received Date : 2019-09-20
  • Revised Date : 2019-11-08
  • Accepted Date : 2019-12-02
  • DOI :https://doi.org/10.7235/HORT.20200010
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
Online Submission
  • scopus
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close