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2019 Vol.37, Issue 5 Preview Page

Research Article

Composition Characteristics of Flavonoids in Citrus Juice

감귤 과즙의 플라보노이드 조성 특성

Young Taek Yang1
,
Ho Bang Kim2
,
Sanghyun Lee3
,
Young Chul Park1*
양 영택1
,
김 호방2
,
이 상현3
,
박 영철1*
1Jeju Special Self-Governing Province Agricultural Research and Extension Services
2Life Sciences Research Institute, Biomedic Co. Ltd.
3Department of Plant Science and Technology, Chung-Ang University
1제주특별자치도 농업기술원
2㈜바이오메딕 생명과학연구소
3앙대학교 식물생명공학과
* Corresponding Author
31 October 2019. pp. 651-662
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Abstract

Flavonoids in juice of 67 citrus genetic resources were analyzed using LC-MS/MS, in order to increase utilization of genetic resources as both breeding and functional materials. Analytical condition was established for ten kinds of flavonoid components known to exist in citrus fruits such as hesperidin by LC-MS/MS, and the analytical method was verified through specificity, linearity, and detection limit. In citrus juice at 80% coloring stage, content of hesperidin and narirutin were generally high, but in sour orange and grapefruit, naringin contents was higher than hesperidin. Total flavonoid content was high in ‘Hayaka’, ‘Sanguinelli’, ‘Grapefruit’, ‘Morita navel’, ‘Kinokuni’ and ‘Mihocore’. And ‘Dangyuja’, ‘Jigak’ and ‘Byungkuyl’ which are Jeju native citrus, has specifically high content of certain flavonoids. These resources could be used as not only sources of functional materials but also breeding materials.

Keywords
analytic method
breeding material
functional material
Jeju native citrus
LC-MS/MS

기능성 및 육종 소재로써 감귤 유전자원의 활용 가치를 높이고자, LC-MS/MS를 이용하여 감귤 67개 유전자원의 과즙 중 플라보노이드 함량을 분석하였다. LC-MS/MS 분석을 위하여 hesperidin 등 감귤에 있다고 알려진 10 종의 플라보노이드 성분의 분석조건을 설정하였고, 특이성, 직선성, 검출한계 등 유효성 검증을 통하여 분석법을 정립하였다. 80% 착색기 감귤 과즙에는 일반적으로 hesperidin과 narirutin 성분 함량이 높았으나, 사우어오렌지와 자몽에서는 naringin 함량이 hesperidin 함량보다 높았다. 총 플라보노이드 함량은 ‘조향’, ‘상귀넬리’, ‘그레이프프루트’, ‘삼전네블’, ‘기주밀감’, ‘미호콜’에서 높게 나타났다. 또한 제주 재래귤인 ‘당유자’, ‘지각’, ‘병귤’ 등은 일부 플라보노이드 성분 함량이 특이적으로 높았다. 따라서 이들 자원들은 기능성 소재뿐만 아니라 육종 소재로 이용될 수 있을 것으로 판단된다.

키워드
분석법
육종 소재
기능성 소재
제주 재래귤
LC-MS/MS
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 37
  • No :5
  • Pages :651-662
  • Received Date : 2019-04-19
  • Revised Date : 2019-07-11
  • Accepted Date : 2019-07-29
  • DOI :https://doi.org/10.7235/HORT.20190065
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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