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2019 Vol.37, Issue 4

Research Article

Evaluation of Pear Pollen Germination through Colorimetric and Cytometric Analyses
Ung Yang1†
,
Seung Gon Wi1†
,
Haesung Kim2
,
Jae-Hak Moon2
,
Seungwon Oh3
,
Min Soo Kim3
,
Sang-Hyun Lee14*
1Asian Pear Research Institute, Chonnam National University
2Department of Food Science & Technology, College of Agriculture and Life Sciences, Chonnam National University
3Department of Statistics, College of Natural Sciences, Chonnam National University
4Department of Horticulture, College of Agriculture and Life Sciences, Chonnam National University
* Corresponding Author
† These authors contributed equally to this work.
31 August 2019. pp. 427-436
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Abstract

This study shows that the germination rate differs based on the method by which pollen is applied to the medium. After 3 h of incubation, the germination rate in the liquid medium (52.65%) was 2.46 times higher than that in the solidified medium (21.37%). This clearly demonstrates that there is a limitation to determining the germination rate in vitro using only the pollen identified from microscopic images, as opposed to all the pollen used in the assay. Colorimetric and cytometric analysis was performed to reduce drawbacks with respect to reliability, subjectivity of counts, and analysis time in the conventional method and improve the accuracy of measurement. The Congo Red (CR) absorbance of newly elongated pollen tubes was gradually increased at pollen concentrations of 1.25 to 5.0 mg·mL-1, and its trend line was expressed by the equation y = 0.127x + 0.0255 with R2 = 0.9938. Although no formula was established for calculating the actual germination rate, the slope of the equation obtained can be interpreted as reflecting the actual germination rate. Differences in pollen distribution between the positive control (PC) and negative control (NC) groups in the Q1-LR quadrant were observed, and the high-density region in the PC group on the dot plot formed a shifted transverse distribution to the right compared to that in the NC group. Although our observations need to be confirmed using larger numbers of pollen samples, we found that the percentage changes in events in the PC groups were expressed as a linear equation (y = 21.302x + 68.972) with a high R-squared value (0.9009). Taken together, these results demonstrate that monitoring the CR absorbance of newly elongated pollen tubes and the changes in pollen distribution along the FSC and SSC parameters will help verify pollen performance.

Keywords
absorbance
accuracy
distribution
equation
monitoring
performance

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 37
  • No :4
  • Pages :427-436
  • Received Date : 2019-02-19
  • Revised Date : 2019-04-22
  • Accepted Date : 2019-04-23
  • DOI :https://doi.org/10.7235/HORT.20190043
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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