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2020 Vol.38, Issue 4 Preview Page

Research Article

Phenotype and Ploidy Analysis of the Colchicine- induced M1 Generation of Echeveria Species
Cabahug Raisa Aone M.1†
,
My Khanh Ha Tran Thi2†
,
Ki-Byung Lim3
,
Yoon-Jung Hwang2*
1Chromosome Research Institute, Sahmyook University
2Department of Convergence Science, Sahmyook University
3Department of Horticultural Science, Kyungpook National University
* Corresponding Author
† These authors contributed equally to this work.
31 August 2020. pp. 522-537
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Abstract

Mutation breeding is an important tool for developing new cultivars in horticulture. Among the many methods of mutation breeding, chemical mutation is highly effective and can be performed easily. Compared to natural breeding methods, higher mutation rates and the faster induction of desirable characteristics have been reported with the use of chemical mutagens. Succulents have recently gained popularity because of their unique geometrical shapes and their ability to survive with minimal watering. Succulents that have peculiar shapes and colors demand higher prices. In this study, we used colchicine, a chemical mutagen, and tested its application on three Echeveria succulent species. A phenotypic evaluation was conducted on the mutant succulents produced from the application of colchicine on propagated leaf cuttings. Phenotypic evaluation included plant parameters and morphological analysis. Ploidy analysis was conducted to confirm the effects of the mutagen treatments. In all selected Echeveria species, the use of colchicine produced mutant species that varied significantly from those of the control; however, treatment concentration and duration varied per species. The phenotypic evaluation revealed that colchicine-mutated plants exhibited compactness, with mutants being generally taller with a thicker but shorter plant diameter compared to that of the control. Mutated plants exhibited prominent changes in color for the a* and b* values. Similarly, changes in leaf shape were observed and were evident at their apexes. These morphological changes are attributed to the change in ploidy level, which was confirmed through stomata and ploidy analysis. Larger stomata size was accompanied by lower stomata density. Based on the flow cytometry analysis, mutated succulents exhibited a 2x-4x complex.

Keywords
chemical mutagen
mutation
ornamental
plant breeding
succulents

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :4
  • Pages :522-537
  • Received Date : 2019-12-16
  • Revised Date : 2020-04-24
  • Accepted Date : 2020-06-07
  • DOI :https://doi.org/10.7235/HORT.20200049
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