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2020 Vol.38, Issue 4 Preview Page

Research Article

Effects of Immersing Treatment Period of CMV Resistant GM and non GM Pepper (Capsicum annuum L.) on Seed Germination

CMV 내성 GM 및 non GM 고추의 침수처리 기간이 종자 발아에 미치는 영향

Kuen Woo Park1*
,
Jun Hong Kim2†
,
Jae Sung Park3
박 권우1*
,
김 준홍2†
,
박 재성3
1Division of Biotechnology, College of Life Science & Biotech., Korea University, Seoul 02841, Korea
2R&D Coordination Division, Rural Development Administration, Jeollabuk-do 54875, Korea
3Division of Food & Resource Economics , Korea University, Seoul 02841, Korea
1고려대학교 생명공학부
2농촌진흥청 연구운영과
3고려대학교 식품자원경제학부
* Corresponding Author
† These authors contributed equally to this work.
31 August 2020. pp. 451-461
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Abstract

This research was performed to provide germination characteristics of environmental risk assessment for genetically modified (GM) CMV-resistant pepper. For the germination test, a CMV-resistant GM line (H-15), its non-GM parental line (P-2377), and two non-GM cultivars (‘Shingang’ and ‘Manita’) were used. The germination of all tested lines was maintained by immersing fully matured red peppers for 60 days in a 200L container where fresh underground water was constantly supplied. The fresh peppers and seeds were placed into plastic bags filled with 3 times the amount of distilled water compared to the volume of peppers and seeds and incubated at 10, 20 and 30°C for 3 months. The germination of pepper fruits from the 4 lines was maintained for 2 months as at 10 and 20°C, but for only 1 month at 30°C. In addition, the germination of seeds from the 4 lines was maintained over 85% for 3 months at 10 and 20°C and 2 months at 30°C. A wintering test was also conducted at the farm water reservoir (7 × 7 × 1.5 m) over 2 years. The germination of the ‘Manita’ line was maintained for only 6 to 7 months and that for the other 3 lines for 7 to 8 months. It was found that the pepper seeds were geminated in the water in June (storage period: 7 months) over 20°C of water temperature. ‘Shingang’ lines with a strong spiciness had the highest germination rate, while ‘Manita’ with low spiciness had the lowest germination rate. H-15 and P-2377 with a moderate germination rate showed no significant difference between them. Since GM peppers in water germinate well for 2 months in autumn and 6 to 8 months in winter, it is important to carefully manage the peppers produced from GM farms to prevent ecological disturbance from storms, heavy rains, and other factors.

Keywords
ecological disturbance
environmental risk assessment
germination ability
germination rate
wintering

본 연구는 CMV 내성 GM 고추의 환경위해성 평가의 기초자료를 얻기 위하여 수행하였다. 공시품종은 CMV내성 GM고추 ‘H-15’와 non GM고추 ‘P-2377(H-15 모본)’, ‘신강’, ‘마니따’등 4품종이었다. 지속적으로 신선한 지하수가 공급되는 콘테이너(200L)에 잘 익은 적색 고추를 가을에 60일 간 침수 처리한 결과, 4품종 모두가 발아력을 유지하였다. 신선한 고추 과실과 종자를 용량의 3배의 증류수를 채운 비닐 지퍼백 속에 넣고 각각 10, 20, 30°C의 인큐베이터에 3개월간 처리하였다. 과실은 4품종 모두 10°C, 20°C에서는 2개월 간 발아력을 유지했으나, 30°C에서는 1개월 동안만 발아력을 가졌다. 종자는 10, 20°C 온도처리에서 4품종 모두 3개월 간 발아율 85% 이상을 유지했다. 그러나 30°C에서는 2개월 간만 발아력을 가졌다. 고추 과일의 2년에 걸친 농장 집수조의 수중 월동시험에서 ‘마니따’만 6 ‑ 7개월, 나머지 3품종은 7 ‑ 8개월 발아력을 유지하였다. 수온이 20°C가 넘는 저장 7개월째에는 고추 종자가 수중 발아하는 것을 발견하였다. 여러 조건에서 매운 맛이 강한 ‘신강’이 가장 높은 발아율을 유지하였고 매운맛이 적은 ‘마니따’가 가장 낮았다. ‘H-15’와 ‘P-2377’은 중간 정도의 발아율을 유지하였으며, 두 품종 간에는 유의 차이가 없었다. GM 고추가 물속에서 가을에는 2개월, 겨울에는 6 ‑ 8개월 간 발아력을 가지므로 GM 농장에서 고추 열매가 폭풍, 폭우, 기타 요인으로 농장 밖으로 나가서 생태계 교란이 나타나지 않게 잘 관리해야 한다.

키워드
생태계 교란
환경위해성 평가
발아력
발아율
월동
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :4
  • Pages :451-461
  • Received Date : 2020-04-20
  • Revised Date : 2020-04-30
  • Accepted Date : 2020-06-08
  • DOI :https://doi.org/10.7235/HORT.20200043
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