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2019 Vol.37, Issue 4 Preview Page

Research Article

Production of Diploid Seedless Watermelon Using Tetraploid Watermelon Pollen

4배체 수박 꽃가루를 이용한 2배체 씨 없는 수박 생산

Taebok Kim1*
,
Jeonghyeon Lim1
,
Juhyung Jeong1
,
Munho Seong1
,
Kabcheol Kim1
,
HyongGwon Jeon2
,
Jaejong Noh3
김 태복1*
,
임 정현1
,
정 주형1
,
성 문호1
,
김 갑철1
,
전 형권2
,
노 재종3
1Watermelon Experiment Station, Jeonbuk Agricultural Research & Extension Services
2Agricultural Environment Division, Jeonbuk Agricultural Research & Extension Services
3National Agrobiodiversity Center, National Institute of Agricultural Biotechnology, Rural Development Administration
1전라북도농업기술원 수박시험장
2전라북도농업기술원 농업환경과
3농촌진흥청 국립농업과학원 농업유전자원센터
* Corresponding Author
31 August 2019. pp. 520-527
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Abstract

Diploid seedless watermelon is produced using soft X-ray irradiated pollen, but production costs are high. This study examined whether soft X-ray-irradiated pollen can be replaced by tetraploid watermelon pollen. The fruit set rate of diploid seedless watermelon differed from that of each tetraploid watermelon line and showed a high correlation with the number of watermelon seeds produced by self-pollination. The fruit set rate was lower when pollens from tetraploid plants were used than when soft X-ray-irradiated pollen was used, but the qualities of watermelons produced were similar. The pollen tube germination rate of tetraploid plants was lower than diploid ones in vivo and in vitro. The shape of tetraploid watermelon pollen was incomplete and uneven compared to that of diploid pollen. Most tetraploid watermelon pollen had no pore, but some had as many as four pores. These results suggest that production of diploid seedless watermelon requires a tetraploid with normal development and high pollen tube germination rate.

Keywords
Citrullus lanatus
fruit set rate
parthenocarpy
pollen tube
pollination

2배체 씨 없는 수박 생산을 위해 연 X-ray 조사 꽃가루를 이용하고 있지만 가격이 비싸다는 단점이 있다. 본 연구는 2배체 씨 없는 수박을 생산함에 있어 4배체 수박의 꽃가루로 연 X-ray 조사 꽃가루를 대체할 수 있는지 살펴보기 위해 수행하였다. 4배체 수박 꽃가루로 2배체 수박 암꽃에 인공수분했을 때의 착과율은 4배체 계통에 따라 큰 차이를 보였으며 4배체 수박을 자가수분해서 채종한 종자량과 높은 상관관계가 있는 것으로 나타났다. 수박의 품질은 연 X-ray 조사 꽃가루로 인공수분 했을 때와 차이를 보이지 않았지만 착과율은 낮았다. 4배체 수박 꽃가루의 꽃가루관 발아율은 in vivo와 in vitro에서 모두 2배체 수박의 꽃가루보다 낮았다. 4배체 수박의 꽃가루 형태는 2배체 수박의 꽃가루에 비해 불완전했고 발아공이 없는 꽃가루가 많았으며 일부 꽃가루에서는 4개의 발아공이 관찰되었다. 4배체 수박 꽃가루를 이용해서 2배체 씨 없는 수박을 생산하기 위해서는 꽃가루의 발달이 정상적이고 꽃가루관 발아율이 높은 4배체를 개발해야 할 것으로 보인다.

키워드
Citrullus lanatus
착과율
단위결실
꽃가루관
수분
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 37
  • No :4
  • Pages :520-527
  • Received Date : 2019-01-07
  • Revised Date : 2019-04-02
  • Accepted Date : 2019-04-12
  • DOI :https://doi.org/10.7235/HORT.20190052
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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