About This Journal

Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provides scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.

 

2018 Impact Factor 0.589

 

  • Research Article

    Fruit Development and Quality of Hot Pepper (Capsicum annuum L.) under Various Temperature Regimes
    Seo-Young Oh, Seok Chan Koh
    Hot pepper (Capsicum annuum L.) originated in South America and is cultivated worldwidely in many temperate regions as well as subtropical and ... + READ MORE
    Hot pepper (Capsicum annuum L.) originated in South America and is cultivated worldwidely in many temperate regions as well as subtropical and tropical regions. During the growing season, various climatic and soil factors, including air temperature, light intensity, precipitation, and soil conditions, affect the growth and development of hot pepper plants. Temperature is one of the major environmental factors affecting processes such as flowering, fruit set, and fruit growth. This study investigated the effects of temperature on the vegetative growth, fruit development, and fruit quality of hot pepper cultivar ‘Muhanjilju’ to develop a cropping system for producing high-quality fruit in temperate regions. The temperature range of 20 - 25°C was favorable for vegetative growth and fruit development. However, a temperature of 30°C reduced fruit pared with that of plants grown at 15°C. In addition, the change in fruit color was advanced significantly at 20 - 25°C and 30°C. A high temperature of 30°C not only reduced the total number of fruit but also caused the formation of short or malformed fruit. Furthermore, the total free-sugar content of red ripe fruit increased significantly at 20 - 25°C, while the capsaicinoid content of red ripe fruit increased as temperatures increased in the range of 15 - 30°C. These results indicate that the temperature range of 20 - 25°C is favorable for development, including fruit set and fruit growth, although it enhanced vegetative growth, and a temperature of 15°C caused short plant height, a small number of branching nodes on the main branch, and elongated fruit. At 20 - 25°C and 30°C, the fruit development period was shortened by 9 - 16 and 22 days, respectively, comthe vegetative growth, fruit development, and fruit quality of hot pepper. - COLLAPSE
    June 2019
  • Research Article

    Growth Promotion Effects of Plant Extracts on Various Leafy Vegetable Crops
    Se Ji Jang, Yong In Kuk
    Though the mechanisms are not yet fully understood, the macro- and micro-nutrients, amino acids, and hormone-like growth substances present in plants (whole ... + READ MORE
    Though the mechanisms are not yet fully understood, the macro- and micro-nutrients, amino acids, and hormone-like growth substances present in plants (whole plants and their extracts) may lead to biostimulatory activities when applied to crops. This study was conducted to determine the growth promotion effects on lettuce (Lactuca sativa L.) of plant extracts obtained by different extraction methods (water, boiling water, fermentation, and ethanol) from 31 agricultural materials. We also determined the growth promotion effects of three other leguminous crop extracts on lettuce. In total, these 31 different agricultural materials produced 82 different extracts. The most effective extracts were water and boiled water extracts of Chinese chive (Allium tuberosum), water extracts of soybean leaves (cvs. Daewon and Haepum), and water, fermentation, and ethanol extracts of soybean stems (cv. Daewon). These induced a 31 - 45% increase in shoot fresh weight of lettuce compared with control plants. Chinese chive, soybean leaf, and soybean stem extracts had a greater impact on shoot fresh weight of lettuce when applied at 20 days after seeding (DAS) than when applied at 30 and 40 DAS. In addition, when extracts from Chinese chive and soybean leaf and stem were used, the shoot fresh weight of lettuce was higher at 7 days after treatment (DAT) than at 14 and 21 DAT. The shoot fresh weight of lettuce was 13 - 15% higher when the selected extracts were applied twice instead of once. Moreover, the growth promotion effects of the selected plant extracts were also observed in endive and broccoli, but not in pak choi and kale. Additionally, the growth promotion of lettuce was higher in response to soybean leaf and stem extracts than extracts of other leguminous crops (cowpea, mung bean, and red bean). The selected extracts showed higher growth promotion effects on lettuce than those of urea and a commercial extract. Overall, the results indicate that the selected plant extracts can be used for growth promotion in organic cultivation of various crops. - COLLAPSE
    June 2019
  • Research Article

    Evaluation of Plug Cell Size and Pinching out Followed by Layering for Mass Propagation Using Stem Cuttings of Virus-Free Sweet Potato Plantlets

    고구마 무병묘의 경삽 대량번식을 위한 플러그 규격과 적심 후 휘묻이 평가

    Na-Ra Lee and Seung-Yeob Lee

    이나라 , 이승엽

    To establish an efficient mass propagation method using stem cuttings of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets, the growth characteristics ...

    고구마 무병묘의 경삽을 이용한 플러그묘 생산과 묘상에서 경삽묘의 대량증식을 위하여, 4 품종(‘맛나미’, ‘신천미’, ‘연황미’, ‘신자미’)을 이용하여 플러그 셀 크기의 영향과 ...

    + READ MORE
    To establish an efficient mass propagation method using stem cuttings of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets, the growth characteristics of four cultivars (‘Matnami’, ‘Shincheonmi’, ‘Yeonhwangmi’, and ‘Shinzami’) influenced by plug cell size and pinching out followed by layering were investigated. Shoot tip cuttings of 5-cm length were grown in plug trays with 50, 72, 105, and 128 cells filled with vermiculite and perlite (1:1, v/v) using the nutrient film technique and the nutrient solution developed by the National Horticultural Experiment Station in Japan for 3 weeks. Shoot tip pinching out (5-cm length) followed by serpentine layerage (SPFSL) was performed after growth for 3 weeks with 10 × 30-cm spacing in nursery soil. The control plantlets were grown with 10 × 10-cm spacing without shoot-tip pinching and layering. Stem growth of plug plantlets was not different among the plug cell sizes up to 2 weeks, but growth after 3 weeks showed the best elongation in 72 cells followed by 50-, 105-, and 128-cell plug trays. Number of leaves, number of roots, and the longest root length were reduced in proportion to cell volume. Fresh weight and dry weight were high in 50- and 72-cell plug trays with more light and soil volume, while they were significantly reduced in 128-cell plug trays. At 6 weeks after transplanting, number of leaves, number of side shoots, fresh weight, and dry weight were significantly increased in SPFSL plants compared to the control. Mean length and number of side shoots (over 5 cm) per plant in SPFSL plants were 1.9 and 1.7 times higher than the control. In this work, the plug cell size for stem cutting was economically suitable in 105-cell plug trays for 2 weeks, and in 72-cell plug trays over 3 weeks. The propagation of stem cutting by SPFSL for farmers was 1.7 times higher than the control.


    고구마 무병묘의 경삽을 이용한 플러그묘 생산과 묘상에서 경삽묘의 대량증식을 위하여, 4 품종(‘맛나미’, ‘신천미’, ‘연황미’, ‘신자미’)을 이용하여 플러그 셀 크기의 영향과 경정절단 파상휘묻이를 하여 생육특성을 조사하였다. 5cm 크기의 경삽묘를 버미큘라이트와 펄라이트 혼합배지(1:1, v/v)를 채운 50, 72, 105, 128구 플러그 트레이(54 × 28 × 5cm)에 삽목하여 NFT 수경베드에서 일본원시액을 공급하여 3주간 육묘하였다. 경정절단 파상휘묻이는 묘상에 10 × 30cm로 이식 후 3주째에 5cm 크기로 경정을 절단하여 휘묻이하였으며, 대조구는 10 × 10cm로 이식 후 경정절단없이 육묘하였다. 경삽묘의 줄기신장은 2주까지는 플러그 셀 크기에 따른 차이가 크지 않았으나, 3주후의 생장은 72구에서 가장 양호하였다. 엽수, 뿌리수 및 뿌리길이 등은 플러그 셀 크기가 작아짐에 따라 감소하였다. 생체중 및 건물중은 상토량과 광조건이 유리한 50구와 72구 플러그에서 양호하였으며, 128구 플러그에서 유의한 감소를 보였다. 경정절단 파상휘묻이 처리는 이식 6주 후에 총 엽수, 곁순수, 평균곁순길이, 생체중 및 건물중 등이 대조구보다 유의하게 증가하였다. 5cm 이상의 곁순수와 평균길이는 경정절단 파상휘묻이 처리에서 대조구보다 1.9배와 1.7배의 유의한 증가를 보였다. 따라서 고구마 무병묘 경삽을 위한 적정 플러그 크기는 육묘기간이 2주 내외일 경우는 105구, 3주 이상일 경우에는 72구에서 적합하였다. 농가에서 경삽묘의 묘상 대량증식은 경정절단 파상휘묻이법으로 육묘하는 것이 증식효율이 1.7배 높았다.

    - COLLAPSE
    June 2019
  • Research Article

    Development of a Functional Plug Tray for Producing High-Quality Strawberry Transplants
    Jean Nepo Ndikumana, Young Hun Lee, and Young Rog Yeoung
    This study was conducted to develop functional plug trays capable of optimizing the root growth environment and inducing early flowering in strawberry ... + READ MORE
    This study was conducted to develop functional plug trays capable of optimizing the root growth environment and inducing early flowering in strawberry plants. Experimental treatments included trays with no drainage slit, two drainage slits, four drainage slits, and a control bottom drainage hole or commercial tray. The effect of the functional plug trays was studied on two strawberry (Fragaria × ananassa Duch.) cultivars (‘Seolhyang’ and ‘Maehyang’) and two different rooting media (commercial medium and peat moss wrapped in a paper pot). The experimental units were arranged in a completely randomized design, with five and four replications for nursery and post-transplanting studies, respectively. Shoot and root growth characteristics and rhizosphere temperature were measured in the nursery study and flowering and yield in the post-transplanting study. Growth parameter results showed that compared to the existing plug trays, the new trays with two and four slits produced transplants of significantly higher quality. These treatments induced lower root temperature in rooting media and plants generally flowered earlier, thus providing higher early and total yields regardless of the media or cultivars used. This effect was further enhanced as the number of drainage holes increased. Thus, an increase in the number of drainage slits in plug trays can lead to higher-quality transplants that can flower and produce fruits earlier than those with poor drainage. - COLLAPSE
    June 2019
  • Research Article

    Assessment of Iranian Rainfed and Seedy Watermelon Landraces as Potential Rootstocks for Enhancing Drought Tolerance
    Ali Parsafar, Jaber Panahandeh, and Davoud Zarehagh
    We conducted this study over the course of two consecutive growing seasons to evaluate the response of watermelon (Citrullus lanatus (Thunb.) Matsum ... + READ MORE
    We conducted this study over the course of two consecutive growing seasons to evaluate the response of watermelon (Citrullus lanatus (Thunb.) Matsum. et Nakai cv. Crimson Sweet) to grafting onto three Iranian rainfed and seedy watermelon landraces collected from Khaje, Sabzevar, and Ashtian, along with ‘Shintoza’ (Cucurbita maxima × Cucurbita moschata) seedlings as a rootstock for watermelon. Soil moisture content [percentage of field capacity (FC)] was determined by time domain reflectometry (TDR). Irrigation was applied in three different levels based on FC as 0.8FC-FC, 0.6FC-0.8FC and 0.3FC-0.6FC by furrow irrigation method. Yield, shoot dry weight, fruit quality, chlorophyll index, phenolic compounds, relative water content (RWC), electrolyte leakage, and proline concentration were measured at the end of both growing seasons. The results showed that the highest fruit yield (9.4 kg) and shoot dry weight (390.8 g) were obtained in watermelons grafted onto Shintoza rootstock in 0.8FC-FC. The lowest fruit yield (1.4 kg) and shoot dry weight (55.6 g) were observed in un-grafted controls and in 0.3FC-0.6FC. Fruit quality in watermelons grafted onto Shintoza rootstock was significantly different compared to the other grafting combinations and the un-grafted control. Chlorophyll index, phenolic compounds, RWC, electrolyte leakage, and proline were significantly affected by irrigation regimes. Shintoza and Sabzevar grafting combinations showed high levels of chlorophyll index, phenolic compounds, and RWC as compared to un-grafted plants and Ashtian grafting combination. - COLLAPSE
    June 2019
  • Research Article

    Suppression of Clover Cyst Nematode Population by the Use of Plastic Mulch in Kimchi Cabbage Cultivation Fields
    Md. Faisal Kabir, Abraham Okki Mwamula, Mungi Jeong, Hyun-Gook Kim, Hyeon-Jeong Ahn, and Dong Woon Lee
    Field experiments were conducted on a Heterodera trifolii infested Kimchi cabbage field in Jungsun, Korea in 2015 (autumn) and 2016 (spring) to ... + READ MORE
    Field experiments were conducted on a Heterodera trifolii infested Kimchi cabbage field in Jungsun, Korea in 2015 (autumn) and 2016 (spring) to investigate the influence of black and transparent plastic mulch as an approach to suppress the nematode population. Initial and final populations of H. trifolii were compared. Both mulches resulted in different final populations of total cysts, cysts with eggs, and eggs/cyst. In 2015, total cysts and cysts with eggs from transparent plastic mulch were significantly lower compared to black plastic mulch and bare soil. However, eggs/cyst showed no significant differences between both mulches. Furthermore, in 2016, all mulches resulted in decreased final populations. Interestingly, eggs/cyst from both mulches and bare soil were significantly lower than 2015. The study thus demonstrated that all plastic mulches could effectively suppress H. trifolii populations but transparent mulch is more effective. - COLLAPSE
    June 2019
  • Research Article

    Changes in Chemical Characteristics of Drainage Solution as Influenced by Years of Root Media Use in Successive Hydroponic Cultivations of ‘Seolhyang’ Strawberries
    Hee Chul Lee, Hyun Sook Kim, Won Suk Jang, Myeong Hyeon Nam, In Ha Lee, Mun Haeng Lee, Tae Il Kim, Eun Mo Lee, and Jong Myung Choi
    Changes in the pH, electrical conductivity (EC), and concentrations of essential nutrients have to be analyzed to enable reuse of the drainage ... + READ MORE
    Changes in the pH, electrical conductivity (EC), and concentrations of essential nutrients have to be analyzed to enable reuse of the drainage solution (DS) in closed hydroponic cultivation of a crop because the chemical characteristics (CC) of drainage vary in relation to the kind of crop grown and the root media used. The objective of this research was to investigate changes in the CC of DS during cultivation of ‘Seolhyang’ strawberries. The root medium is composed of coir dust, peat moss, and perlite in the ratio 6:2:2 (v/v/v), and the composition of the nutrient solution applied for crop cultivation is N, P, K, Ca, and Mg at 15, 5, 7, 6, and 2 mg·L-1, respectively. The changes in the CC of the DS as well as the growth, yield, and fruit quality were investigated in relation to the number of years the root media used. The pH of the DS decreased as the number of years of root medium usage increased. The ECs of DS in all treatments were in the range of 0.8 to 1.5 dS·m-1 during the growing season and tended to rise as the number of years of usage in the root medium increased. The concentrations of NO3 and Ca in the DS ranged from 200 to 600 mg·L-1 and 52 to 176 mg·L-1, respectively. The K concentration in the DS was in the range of 104 to 221 mg·kg-1 in all treatments during the growing season. The concentrations of P and Mg in the DS were 15 to 60 mg·L-1 and 18 to 48 mg·L-1, respectively. As the number of years of root media usage increased, the concentrations of NO3, P, Ca, and Mg tended to rise, whereas that of K tended to decrease. The different treatments with varying number of years of root medium usage did not lead to significant differences in plant growth, tissue nutrient content, budding and flowering date, and total yield. The results from this research, the changes in nutrient composition of the DS and their effect on tissue nutrient content, can be used to modify the nutrient composition in the DS when the solution is reused in closed hydroponic cultivation of ‘Seolhyang’ strawberries. - COLLAPSE
    June 2019
  • Research Article

    Effect of Application Timing of Deastringency and 1-Methylcyclopropene Treatments on Fruit Quality Attributes and Physiological Disorders of Two Astringent Persimmon Cultivars during Cold Storage
    Jingi Yoo, In-Kyu Kang, Seulgi Ryu, Nay Myo Win, and Jinwook Lee
    Astringent persimmon (Diospyros kaki Thunb.) fruit are treated with high levels of CO2 or alcohol treatments to remove bitterness, but additional treatments ... + READ MORE
    Astringent persimmon (Diospyros kaki Thunb.) fruit are treated with high levels of CO2 or alcohol treatments to remove bitterness, but additional treatments are needed to maintain fruit firmness for long-term cold storage. Currently, 1-methylcyclopropene (1-MCP) is widely employed to maintain fruit firmness during cold storage. The objective of this study was to evaluate the effects of application timing of a combination of deastringency and 1-MCP treatments on fruit quality attributes and the incidence of physiological disorders in the cold-stored astringent persimmon fruit of two cultivars, ‘Tonewase’ and ‘Sangjudungsi’. Fresh weight loss gradually increased in ‘Tonewase’, regardless of the timing of the deastringency and 1-MCP treatment. However, the 1-MCP treatment alone resulted in the smallest loss of fresh weight compared with the other treatments in ‘Sangjudungsi’. Fruit firmness was highly maintained by the 1-MCP treatment, regardless of cultivar and application timing. Furthermore, the soluble solids content (SSC) was not affected by the 1-MCP treatment, but was significantly reduced by the deastringency treatment. The SSC was highly retained in ‘Sangjudungsi’. The variation in various color variables was greater in ‘Sangjudungsi’ fruit than in ‘Tonewase’ fruit. Compared with the other treatments, the combination of 1-MCP and deastringency treatments significantly reduced all color variables at both of the peel regions of ‘Tonewase’ fruit. Fruit softening, decay, and wilting rates were highest in the combined 1-MCP and deastringency treatments in ‘Tonewase’ fruit. On the other hand, ‘Sangjudungsi’ was relatively less susceptible to physiological disorders during cold storage. Overall, our results show that astringent persimmon cultivars require individually-tailored management techniques immediately before cold storage to retain fruit quality. - COLLAPSE
    June 2019
  • Research Article

    Effect of Different CO2 Deastringency Application Timing on Fruit Quality Attributes and Physiological Disorders in Cold-Stored ‘Sangjudungsi’ Persimmon Fruit
    Nay Myo Win, Jingi Yoo, Jinwook Lee, Hee-Young Jung, and In-Kyu Kang
    The effects of different application timing of CO2 deastringency treatment on fruit quality attributes and physiological disorders of ‘Sangjudungsi’ persimmon fruit during ... + READ MORE
    The effects of different application timing of CO2 deastringency treatment on fruit quality attributes and physiological disorders of ‘Sangjudungsi’ persimmon fruit during cold storage were evaluated. Treatment with an application of 95% CO2 at harvest ensured the complete removal of astringency after one or two months of cold storage. The deastringency treatment greatly reduced flesh firmness, and early CO2 application caused the lowest flesh firmness at the end of cold storage. The soluble solids content (SSC) was also decreased after CO2 application, while fruit treated later with CO2 had lowest SSC, compared with the other CO2 application timings. Weight loss and respiration rate both increased in deastringency-treated fruit. Peel color variables, as measured using the CIELab color space, were also affected by the treatment. The changes in peel color variables were more detectable in the calyx-end regions than in the equatorial regions. Fruit treated with CO2 one-month after harvest were observably different in peel color variables. Flesh browning was also detected in fruit treated one-month after harvest. The decrease in astringency following CO2 treatment led to increased incidence of flesh browning and fruit softening at the end of the cold storage period. Peel blackening was also detected in early deastringency-treated fruit at the end of cold storage. This study suggested that earlier CO2 application may decrease flesh firmness and increase the incidence and severity of physiological disorders in ‘Sangjudungsi’ persimmon fruit during cold storage. - COLLAPSE
    June 2019
  • Research Article

    Genetic Analysis and Vase Life Characteristics of a New Bright-Pink Gerbera Cultivar ‘Pink Garden’ with Double Flowers

    화색이 선명한 핑크색 겹꽃 거베라 ‘핑크가든’ 육성과 유전적 분석 및 수확 후 절화 특성

    Yong-Mo Chung and Jung-Soo Lee

    정용모, 이정수

    A new bright-pink gerbera (Gerbera hybrida Hort.) cultivar, ‘Pink Garden’, was released by the Flower Research Institute of Gyeongsangnam-do Agricultural Research & Extension ...

    경남농업기술원 화훼연구소에서 핑크색 대륜계의 ‘핑크라이트’를 모본으로 하고 핑크색 대륜계의 ‘메피스토’를 부본으로 인공교배를 실시하여, 선명한 핑크색의 겹꽃으로 절화용 거베라 ‘핑크가든(Pink ...

    + READ MORE
    A new bright-pink gerbera (Gerbera hybrida Hort.) cultivar, ‘Pink Garden’, was released by the Flower Research Institute of Gyeongsangnam-do Agricultural Research & Extension Service in 2013. ‘Pink Garden’ was developed in Changwon on 2007 and is a cross between ‘Pink Light’, a semi-double-type gerbera cultivar with pink flowers, and ‘Mephysto’, a semi-double-type cultivar with purple flowers. The characteristic trials for selection were conducted on 2008. ‘Pink Garden’ has large, double-type flowers with pink (RHS, 55B) ray florets. It also has a steady flower shape, middle-strong peduncles, and a vase life of 13.5 days. The average flower yield of ‘Pink Garden’ was determined in greenhouse trials conducted from 2009 to 2010 and was determined to be 49.5 stems per plant per year. ‘Pink Garden’ was revealed to be a new cultivar based on random amplified polymorphic DNA analysis and was registered (Grant No. 4575) in the Korea Seed and Variety Service for commercialization in 2013.


    경남농업기술원 화훼연구소에서 핑크색 대륜계의 ‘핑크라이트’를 모본으로 하고 핑크색 대륜계의 ‘메피스토’를 부본으로 인공교배를 실시하여, 선명한 핑크색의 겹꽃으로 절화용 거베라 ‘핑크가든(Pink Garden)’을 육성하였다. 인공교배에서 획득한 실생 계통으로부터 선발하여 조직배양과 재배를 통해 개체 증식과 생육 및 특성 검정, 농가 실증, 절화수명 등을 평가하였다. ‘핑크가든’의 화색은 선명한 핑크색(RHS 55B)으로 꽃의 직경이 11.5cm 정도의 대륜화이며, 화경장은 58.3cm 정도였다. 개화소요일수가 89.6일 정도이며, 절화 수량도 49.5개로 대조품종보다 개화가 빠르고 수량도 많았다. 절화수명에 있어서도 13.5일로 대조품종보다 우수하였으며, 절화보존 시 꽃 크기 변화 등이 적고, 외형 등의 변화가 대조품종보다 우월하여 절화로서 높은 관상가치를 보였다. 또한 농가 실증이나 기호도 평가 시 잘 조화된 절화 품종으로 평가 받았다. RAPD 분석에서 육성품종이 모본과 부본의 밴드 패턴이 ‘핑크가든’에 모두 나타나, 양친 간 교배에 의해 육성된 품종임을 확인할 수 있었다. ‘핑크가든(Pink Garden)’은 2013년에 신품종으로 품종보호등록(품종보호 제4575호)이 되었으며, 절화용 거베라로서 농가소득 창출에 기여할 수 있을 것으로 기대된다.

    - COLLAPSE
    June 2019
  • Research Article

    Development of Analytical Method for Fenamidone Residues in Horticultural Crops Using HPLC-UVD/MS

    HPLC-UVD/MS를 이용한 원예작물의 Fenamidone 잔류 분석법 개발

    Myoung-Gun Choung and In-Kyu Kang

    정명근, 강인규

    This experiment was conducted to establish an analytical method for residues of fenamidone, such as imidazole fungicide, in horticultural crops using HPLC-UVD ...

    본 연구는 HPLC-UVD/MS를 이용하여 원예작물의 균류 호흡억제 효과를 나타내는imidazolinone계 살균제 fenamidone의 잔류 분석법을 확립하고자 원예작물 중 사과, 고추, 배추 및 오이를 ...

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    This experiment was conducted to establish an analytical method for residues of fenamidone, such as imidazole fungicide, in horticultural crops using HPLC-UVD/MS. Fenamidone residue was extracted with acetone from representative samples of four horticultural crops which comprised apple, green pepper, kimchi cabbage, and cucumber. The extract was purified by optimized dichloromethane liquid-liquid partition and florisil column chromatography. On an octadecylsilyl column in HPLC, fenamidone was successfully separated from co-extractives of sample, and limit of quantitation (LOQ) of fenamidone was 0.04 mg·kg-1 as verified by the recovery experiment. Mean recoveries ranged from 85.5% to 97.9% in four representative horticultural crops. The coefficients of variation were all less than 10%, irrespective of sample types and fortification levels. A confirmatory method using LC/MS with selected-ion monitoring technique was also provided to clearly identify the suspected residue. The proposed method can be used as a reliable quantitative analytical method that accepts international analytical criteria in terms of analytical sensitivity, reproducibility, ease of operation, recovery rate, and analysis error.


    본 연구는 HPLC-UVD/MS를 이용하여 원예작물의 균류 호흡억제 효과를 나타내는imidazolinone계 살균제 fenamidone의 잔류 분석법을 확립하고자 원예작물 중 사과, 고추, 배추 및 오이를 대상으로 하였다. 시료에 함유된 fenamidone 성분은 아세톤으로 추출하고, dichloromethane 액-액 분배 및 florisil 흡착크로마토그래피법으로 정제하여 HPLC로 분석하였다. C18 칼럼을 이용한 HPLC 분석 시 fenamidone 성분은 작물 유래 추출성분과 완전히 분리되었고, 회수율 시험을 통한 fenamidone의 정량한계(LOQ)는 0.04mg·kg-1 이었다. 4종의 원예작물에 대한 평균 회수율은 85.5 - 97.9%를 나타내었고, 반복 간 변이계수(CV)는 원예작물의 종류 및 처리농도에 관계 없이 10% 미만을 나타내었다. 또한 LC/MS SIM을 이용한 재확인 과정에서도 잔류성분을 재확인 할 수 있었다. 이상의 결과로 신규 fenamidone 분석법은 분석감도, 재현성, 작업의 편이성, 회수율 및 분석오차 면에서 국제적 분석기준을 충족하는 신뢰성이 확보된 정량 분석법으로 사용 가능할 것이다.

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    June 2019