• Research Article

    Characterization of Pollen Development in Staminate Kiwifruit (Actinidia sp.) Cultivars
    Eun Ui Oh, Seung Yong Jeong, Hyeong Ho Kang, and Kwan Jeong Song
    This study was conducted to determine the timing of flower harvest for pollen collection and preparation in kiwifruit. Here the pollen development ... + READ MORE
    This study was conducted to determine the timing of flower harvest for pollen collection and preparation in kiwifruit. Here the pollen development of two staminate cultivars, ‘Bohwa’ and ‘Chieftain’, which are the major pollinizers used in the region of Jeju in Korea, was investigated. These two staminate cultivars showed a similar tendency in histological pollen development, except for the time of anther dehiscence. The highest pollen quantity was obtained at the 4th developmental stage; thereafter, the quantity of pollen decreased by 23% and 38% at the 5th developmental stage in ‘Bohwa’ and ‘Chieftain’, respectively. The pollen viability of ‘Bohwa’ was slightly higher than that of ‘Chieftain’ in both 1% iodine potassium iodide and fluorescein diacetate staining with a small increase according to progression in flower development from the 1st to the 5th stage. Pollen germination also increased as flower development advanced, and higher pollen germination of more than 60% occurred in the 4th developmental stage of both staminate cultivars. The results indicate that the appropriate timing for pollen collection might be the 4th developmental stage (full balloon stage) in ‘Bohwa’ and ‘Chieftain’ according to the amount of pollen production, viability, and germination. - COLLAPSE
    February 2020
  • Research Article

    Enhanced Drought Tolerance in Chinese Cabbage (Brassica campestris L.) Seedlings Upon Pretreatment with Exogenous Salicylic Acid
    Ye Ryung Cha, Seongmin Kim, Jic Hyun Lee, and Ie-Sung Shim
    Salicylic acid (SA) is an important signal transduction molecule that helps plants overcome external stress conditions. Drought stress can strongly impact plants ... + READ MORE
    Salicylic acid (SA) is an important signal transduction molecule that helps plants overcome external stress conditions. Drought stress can strongly impact plants during cultivation. In this study, we investigated the effects of pretreating Chinese cabbage seedlings with exogenous SA during drought stress through physiological and biochemical analyses. The study lasted for 39 days, including a period of 23-day during which Chinese cabbage seedlings were pretreated with SA. Plants were pretreated three or six times with SA (10-4 M) or left untreated (control), and subjected to a 12-day drought period. Leaf samples were harvested before (day 0) and on days 3, 6, 9, and 12 of the drought period. Malondialdehyde (MDA) content, photosynthesis rate, chlorophyll fluorescence, total chlorophyll content, and antioxidant enzyme (catalase, ascorbate peroxidase, glutathione reductase) activities were determined during the drought period. Pretreatment with SA led to enhanced drought tolerance in Chinese cabbage seedlings. Pretreating seedlings six times was found to be more efficacious than three times. SA pretreatment reduced MDA synthesis and prevented leaf rolling and leaf scorching. Additionally, SA pretreatment preserved the photosynthetic apparatus and counteracted the reduced activity of antioxidant enzymes. These results demonstrate that pretreatment with exogenous SA protects Chinese cabbage seedlings from oxidative stress and promotes their growth. We thus conclude that pretreatment with exogenous SA can enhance drought tolerance in Chinese cabbage seedlings. - COLLAPSE
    February 2020
  • Research Article

    Effect of Pre-harvest Calcium Chloride and Chitosan Treatments on Fruit Quality of ‘Benica’ Sweet Cherry

    수확 전 염화칼슘 및 키토산 처리가 ‘베니카’ 체리 과실 특성에 미치는 영향

    Young-Suk Lee, Mi-Geon Cheon, Yong-Mo Chung, Hee-Dae Kim, H.M. Prathibhani C. Kumarihami, and Jin-Gook Kim

    이영숙, 천미건, 정용모, 김희대, 헤라쓰 무디얀셀라지, 김진국

    In this study, the effects of pre-harvest application of calcium chloride and chitosan, separately and in combination, on fruit quality characteristics of ...

    본 연구는 수확 전 염화칼슘과 키토산 처리에 의한 ‘베니카’ 체리의 열과 억제와 저장성 향상 효과를 구명하고자 실시하였다. ‘베니카’ 체리(6년생)의 만개 ...

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    In this study, the effects of pre-harvest application of calcium chloride and chitosan, separately and in combination, on fruit quality characteristics of ‘Benica’ sweet cherry fruit during postharvest storage was investigated. Six year old ‘Benica’ sweet cherry plants were foliar sprayed once with 0.5% calcium chloride at 30 days after full bloom. Chitosan was foliar sprayed at 100 ppm, alone or in combination with 0.1% calcium chloride five times at five day intervals starting from 30 days after full bloom. Untreated plants served as control. Fruits were harvested at 53 days after full bloom, packaged in transparent plastic boxes, and stored separately at either 4°C or 22°C. The fruit quality characteristics were evaluated after 5 and 7 days of storage. The cherry fruit cracking ratio was recorded after immersed the fruit in water for 24 hours at room temperature (25°C). Fruit firmness and soluble solids content were higher with 0.5% calcium chloride and 100 ppm chitosan, alone or in combination with 0.1% calcium chloride, compared to the control. The fruit cracking ratio was lower with the combination of 0.1% calcium chloride and 100 ppm chitosan. Based on scanning electron microscope images of ‘Benica’ sweet cherry fruit skin, chitosan- treated fruit had smoother skin than the control, which may be due to the formation of a protective layer formed by chitosan on the fruit skin. In summary, pre-harvest treatment of calcium chloride and chitosan was effective in reducing the cracking and improving the firmness of ‘Benica’ sweet cherry fruit.


    본 연구는 수확 전 염화칼슘과 키토산 처리에 의한 ‘베니카’ 체리의 열과 억제와 저장성 향상 효과를 구명하고자 실시하였다. ‘베니카’ 체리(6년생)의 만개 후 30일에 0.5% 염화칼슘을 한번 처리, 100mg·L-1 키토산을 만개 후 30일부터 5일 간격 5번 처리, 0.1% 염화칼슘과 100mg·L-1 키토산 혼용액을 만개 후 30일부터 5일 간격 5회 처리 그리고 무처리 하였다. 각 처리별 과실특성을 조사하였고 25°C 상온의 물에 24시간 동안 체리를 침지 한 후 시간에 따른 열과 비율을 조사하였다. 가용성 고형물 함량과 경도는 0.5% 염화칼슘 처리, 100mg·L-1 키토산 처리, 0.1% 염화칼슘 + 100mg·L-1 키토산 혼용액 처리가 무처리 보다 높은 경향이었다. 열과 비율은 0.1% 염화칼슘 + 100mg·L-1 키토산 혼용액 처리가 낮은 경향이었다. 수확 후 ‘베니카’ 체리의 과피를 전자현미경으로 관찰하였을 때 키토산을 처리한 처리구의 과피가 무처리 보다 매끈하였다. 키토산 처리구의 과피가 매끈한 것은 과피에 보호막이 형성된 것으로 여겨졌다. 수확 전 염화칼슘과 키토산 처리는 ‘베니카’ 체리의 경도를 향상시키고 열과를 줄이는데 효과가 있는 것으로 판단되었다.

    - COLLAPSE
    February 2020
  • Research Article

    Changes in Fruit Characteristics and Expression of Ripening-Related Genes in ‘Madoka’ Peaches Treated with 1-Methylcyclopropene and Aminoethoxyvinylglycine

    1-MCP 및 AVG 수체처리에 따른 ‘마도카’ 복숭아 과실의 저장 중 특성 변화 및 성숙 관련 유전자 발현

    Dan Bi Lee, Guk Jin Lee, Seung Heui Kim, In Kyu Kang, Seong Jin Choi, and Hae Keun Yun

    이단비, 이국진, 김승희, 강인규, 최성진, 윤해근

    Changes of the characteristics were investigated during storage of peach fruits following foliar application of 1-methylcyclopropene (1-MCP, 120 mg∙L-1 and 240 ...

    1-Methylcyclopropene(1-MCP, 120mg·L-1, 240mg·L-1)와 aminoetoxyvinylglycine(AVG, 75mg·L-1) 및 AVG(75mg·L-1) + 1-MCP(240mg·L-1)를 수확 3주 전에 ...

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    Changes of the characteristics were investigated during storage of peach fruits following foliar application of 1-methylcyclopropene (1-MCP, 120 mg∙L-1 and 240 mg∙L-1), aminoetoxyvinylglcine (AVG, 75 mg∙L-1), and a combination of 1-MCP (240 mg∙L-1) and AVG (75 mg∙L-1) onto peach trees 3 weeks before harvesting. There were no significant differences in fruit characteristics including total soluble solids, acid content, and Hunter’s value a between control and 1-MCP- or AVG-treated fruits of ‘Madoka’ peaches during ambient (25°C) and cold storage (12°C). However, we observed a delayed decrease in firmness, as well as inhibition of ethylene production and respiration during storage of peach fruits after foliar application of 1-MCP, AVG, and AVG combined with 1-MCP in 2017 and in 2018 compared to untreated peach fruits. Quantitative real-time PCR using RNA isolated from peach fruits revealed significantly inhibited expression of sugar accumulation-related genes (polygalacturonase, PpPG and PpPGL; and lipoxygenase, PpLOX), cell wall softening-related gene (pectin methylesterase, PpPME), ethylene biosynthesis-promoting genes (ACC synthase, PpACS1; and ACC oxidase, PpACO), and genes responsive to ethylene receptors (ethylene insensitive protein, PpEIN; and ethylene response sensor 1, PpERS1) in fruits treated with 1-MCP or AVG after harvest. This suggests that foliar application with 1-MCP or AVG can extend the shelf-life of peaches through inhibition of ethylene production and respiration. These results are informative for the development of technology necessary to extend shelf-life, which may ultimately lead to increased consumption and export to foreign countries of peaches produced in Korea.


    1-Methylcyclopropene(1-MCP, 120mg·L-1, 240mg·L-1)와 aminoetoxyvinylglycine(AVG, 75mg·L-1) 및 AVG(75mg·L-1) + 1-MCP(240mg·L-1)를 수확 3주 전에 처리하여 중생종 ‘마도카’ 복숭아의 저장 중 특성 변화를 조사하였다. 과실 수확 후 상온 저장과 저온 저장 중 가용성 고형물 함량, 산 함량, 과피색 변화 등의 과실특성은 처리와 무처리 간의 차이가 나타나지 않았다. 수확 직후의 과실에서도 1-MCP와 AVG를 처리한 과실에서는 경도가 높았으며, 저장 기간이 경과하면서 1-MCP와 AVG를 처리한 과실에서 연화가 억제되었다. 에틸렌 발생은 수확 직후에도 1-MCP와 AVG를 처리한 과실에서 무처리구에 비해 현저하게 낮았으며, 상온 저장과 저온저장 조건에서 모두 저장 일수가 경과함에 따라 무처리구에서 가장 높았으며 1-MCP와 AVG를 처리한 모든 과실에서 낮게 나타났다. 1-MCP와 AVG 를 처리한 후 저장 중인 과실에서 저장 일 수가 경과함에 따라 상온 저장과 저온 저장 중인 과실에서 모든 처리구에서 호흡률이 감소하였다. 1-MCP와 AVG 를 처리한 과실에서 RNA를 분리하여 real-time-PCR을 수행한 결과 당대사에 관여하는 유전자(PpPG, PpPGL, PpLOX), 세포벽 연화와 관련된 유전자(PpPME)는 물론 에틸렌 합성(PpACS1, PpACO) 과 수용체의 반응에 관여하는 유전자(PpEIN, PpERS1)의 발현이 억제되었다. 1-MCP와 AVG의 복숭아 수체 처리에 의해 과실의 에틸렌 합성과 호흡의 감소를 유도함으로써 저장 기간의 연장을 가져올 수 있을 것으로 여겨진다. 이러한 결과는 복숭아의 보구력을 향상시키고 저장 기간을 연장하기 위한 기술 개발은 물론 복숭아의 소비 촉진과 해외 수출 확대에도 크게 기여할 것이다.

    - COLLAPSE
    February 2020
  • Research Article

    Study on the Characteristics of Growth, Yield, and Pharmacological Composition of a new Glycyrrhiza Variety Licorice ‘Wongam (Glycyrrhiza glabra × Glycyrrhiza uralensis)’ in Temperature Gradient Tunnel and Suitable Cultivation Area of Korean

    온도구배터널에서 기온상승에 따른 신품종 감초 원감 (Glycyrrhiza glabra × Glycyrrhiza uralensis)의 생육, 수량, 약리성분 특성 및 국내 재배적지에 관한 연구

    Yong Il Kim, Jeong Hoon Lee, Tae-Jin An, Eun Song Lee, Woo Tae Park, Young-Guk Kim, and Jae Ki Chang

    김용일, 이정훈, 안태진, 이은송, 박우태, 김영국, 장재기

    Licorice is a semi-desert plant that is difficult to grow under Korean climatic conditions. In order to increase the adaptability of licorice ...

    감초는 반사막성 식물로 국내 기후조건에서는 재배가 어렵다. 감초의 국내 재배 적응성을 높이기 위하여 최근 농촌진흥청에서 감초 신품종 ‘원감(Wongam, Glycyrrhiza glabra × Glycyrrhiza ...

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    Licorice is a semi-desert plant that is difficult to grow under Korean climatic conditions. In order to increase the adaptability of licorice in Korea, the Rural Development Administration (RDA) has recently developed a new variety of licorice, ‘Wongam (Glycyrrhiza glabra × Glycyrrhiza uralensis)’. In this study, the growth, yield, and pharmacological properties of the Wongam variety were investigated in accordance with temperature conditions to evaluate the suitability of the Wongam variety for cultivation. In the temperature gradient tunnel (TGT) atEumseong, Chungbuk, when the temperature was raised to 0°C ‑ 1.5°C higher than the local temperature in 2017, the average height of the Wongam variety was 121.6 cm and the average yield (10a) was 878.1 kg. When the temperature was raised to be 3.0°C ‑ 4.5°C above the local temperature, the average height was 146.5 cm and the average yield (10a) was 1,484.6 kg, anincrease of 69.1%. When the temperature was raised to be 4.5°C ‑ 5.9°C above the local temperature, the average height decreased to 132.5 cm and the yield decreased 8.0% to 1,366.3 kg. Glycyrrhizin content of Wongam roots decreased steadily from 0.98% to 0.87% as the temperature in the TGT increased. The overall average content was 0.91%, which is presumed to be because of a lack of accumulation due to short-term cultivation. The results of this study showed thatthe production of the Wongam variety increased as the temperature was raised to about 4.5°C higher than the local temperature based on the 2017 temperature of Eumseong, Chungbuk, Korea. Therefore, if the current cultivation area formed in Jecheon in Chungbuk is extended to the south central region, the productivity of this cultivar can be greatly improved. In addition, the cultivated years should be extended to enhance glycyrrhizin content up to the 2.5% of the Korean Pharmacopoeia.


    감초는 반사막성 식물로 국내 기후조건에서는 재배가 어렵다. 감초의 국내 재배 적응성을 높이기 위하여 최근 농촌진흥청에서 감초 신품종 ‘원감(Wongam, Glycyrrhiza glabra × Glycyrrhiza uralensis)’을 개발하였다. 본 연구에서는 원감 품종의 재배적지 평가를 위하여 온도조건에 따른 생육, 수량, 약리성분 특성을 조사하였다. 충북 음성 소재 온도구배터널에서 2017년 현지기온 대비 0 ‑ 1.5°C 상승시 원감 품종의 평균 초장은 121.6cm, 평균 수량(10a)은 878.1kg이었다. 3.0 ‑ 4.5°C까지 상승시키자 평균 초장은 146.5cm, 평균 수량(10a)은 1,484.6kg으로 69.1%까지 증가하였다. 외기온도 대배 4.5 ‑ 5.9°C까지 상승시키자 평균 초장은 132.5cm로 감소하였고, 수량도 1,366.3kg으로 8.0% 감소하였다. Glycyrrhizin 함량은 터널 내 온도가 올라갈수록 0.98%에서 0.87%로 연속해서 감소하였다. 전체 평균 함량은 0.91%로 단기재배에 따른 축적부족이 원인인 것으로 추정된다. 본 연구결과, 2017년 충북 음성 기준 약 4.5°C까지 온도가 상승할수록 원감 품종의 생산량이 증가하였으므로 현재 충북 제천을 중심으로 형성된 재배지역을 중남부 지역까지 확대할 경우 이 품종의 생산성을 향상시킬 수 있을 것으로 사료된다. 또한 대한약전 기준치인 2.5%까지 글리시리진 함량을 증진시키기 위해서는 재배기간 연장을 고려해 볼 필요가 있다.

    - COLLAPSE
    February 2020
  • Research Article

    Growth and Mineral Content of Southern-type Garlic Grown in Volcanic and Non-volcanic Ash Soils on Jeju Island

    제주지역의 화산회토양과 비화산회토양에서 재배된 난지형 마늘의 생장과 무기성분 함량

    Seo Young Oh and Seok Chan Koh

    오서영, 고석찬

    The growth and mineral content of garlic (Allium sativum L.) grown in different types of soil on Jeju Island, including black volcanic ...

    본 연구는 고품질의 마늘을 수확하기 위해서 필요한 토양 개선과 작물 재배 시 관리 방법을 평가하기 위하여 제주지역의 흑색 화산회토양(BS), 농암갈색 화산회토양 ...

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    The growth and mineral content of garlic (Allium sativum L.) grown in different types of soil on Jeju Island, including black volcanic ash soil (BS), very dark-brown volcanic ash soil (VDBS), and dark-brown non-volcanic ash soil (DBS), were investigated to evaluate soil–crop system management, which is required to increase crop quality and productivity. Examining the soil physicochemical properties, the organic matter and total nitrogen contents were higher, while the available P2O5 and Mg contents were lower, in the volcanic ash soils than in the non-volcanic ash soil. During the growing stage, plant height and pseudo stem diameter were greater in garlic grown in VDBS or DBS compared to BS. At harvest time, bulb diameter and length, the number of cloves per bulb, and clove weight were greater in VDBS or DBS than in BS. In addition, in BS, shoot growth, bulb development, and clove differentiation were abnormal. The total N, P, K, Ca, Fe, Mn, Zn, and Cu contents in the bulbs were also higher in garlic grown in DBS or VDBS compared to BS. Specifically, the K, Mn, Zn, and Cu contents increased significantly in garlic grown in DBS. These results suggest that soil physicochemical properties affected the growth and development of garlic, and that the cloves should be grown in VDBS or DBS to increase the productivity of high-quality garlic with large, mineral-rich bulbs.


    본 연구는 고품질의 마늘을 수확하기 위해서 필요한 토양 개선과 작물 재배 시 관리 방법을 평가하기 위하여 제주지역의 흑색 화산회토양(BS), 농암갈색 화산회토양(VDBS)과 암갈색 비화산회토양(DBS)에서 마늘(Allium sativum L.)을 재배하고 생육특성과 무기성분 함량을 조사하였다. 토양 유형별 물리화학적 특성을 살펴보았을 때 유기물과 질소 함량은 화산회토양(흑색 및 농암갈색)이 비화산회토양(암갈색)보다 더 높았고, 유효인산(Av. P2O5)과 마그네슘(Mg) 함량은 비화산회토양이 화산회토양보다 더 높았다. 생육기간동안 지상부의 길이와 엽초경은 농암갈색 화산회토양과 암갈색 비화산회토양에서 더 빠르게 생장하였으며, 흑색 화산회토양에서는 저조하였다. 수확기 인경 두께와 길이도 농암갈색 화산회토양과 암갈색 비화산회토양에서 더 잘 발달하였으며, 흑색 화산회토양에서는 인경 발달이 가장 저조하였다. 그리고, 인경 당 인편 수는 농암갈색 화산회토양과 암갈색 비화산회토양에서는 각각 7개와 6개로 많고, 흑색 화산회토양에서는 4개에 불과하였으며, 인편 무게도 농암갈색 화산회토양에서 수확한 마늘이 더 무거웠다. 그러나 흑색 화산회토양에서는 지상부의 생장뿐만 아니라 인편 분화 및 비대가 정상적으로 이루어지지 않았다. 인경의 T-N, P, K, Ca, Fe, Mn, Zn, Cu 함량은 암갈색 비화산회토양과 농암갈색 화산회토양에서 재배하였을 때 높은데 반하여, 흑색 화산회토양에서는 낮았다. 특히, 암갈색 비화산회토양에서 재배되었을 때에 K, Mn, Zn, Cu 함량이 크게 증가하였다. 이상의 결과를 토대로 토양의 물리화학적 특성이 마늘의 생육과 인경 발달에 영향을 미치는 것을 알 수 있었으며, 높은 수확량과 고품질의 마늘을 생산하기 위해서는 농암갈색 화산회토양과 암갈색 비화산회토양에서 마늘을 재배하는 것이 바람직한 것으로 보인다.

    - COLLAPSE
    February 2020
  • Research Article

    Comparison of Chlorophyll Fluorescence and Photosynthesis of Two Strawberry Cultivars in Response to Relative Humidity
    Hyo Gil Choi and Ho Jeong Jeong
    The aim of this study was to evaluate growth response of two greenhouse-grown strawberry cultivars grown under two different relative humidity (RH ... + READ MORE
    The aim of this study was to evaluate growth response of two greenhouse-grown strawberry cultivars grown under two different relative humidity (RH) regimes using photo-physiological measurements, such as photosynthetic rate and photosynthesis efficiency (with the initial chlorophyll fluorescence curve part of 0 ‑ 30millisec (OJIP) transients). The two different RH regimes (< 40% and > 60% RH) were maintained during daylight hours from November 20, 2018 to December 20, 2018. After 15 days of controlled humidity treatment, we found significant differences in photosynthetic rates, stomatal conductance, energy flux trapped by one active photosystem II reaction center (TRo/RC), and rate of electron transport flux of photosystem II reaction center (ETo/RC) values relative to cultivar type and RH. However, after 30 days of humidity treatment, the significant differences in these photosynthesis related parameters were responses related to RH treatment effects regardless of the cultivars. Both ‘Arihyang’ and ‘Keumsil’ cultivars grown under > 60% RH (during the day) showed higher root activity, reflecting multiple turnover of QA reductions (Ss), TRo/RC, and ETo/RC values than when grown under < 40% RH. In addition, relative variable chlorophyll fluorescence at 2millisec (Vj) and relative variable chlorophyll fluorescence at 30millisec (Vi) values were higher in the ‘Arihyang’ cultivar (regardless of RH) than in the ‘Keumsil’ cultivar. We conclude that maintaining high relative humidity inside greenhouses during the cold season (when RH is naturally low) will optimize strawberry fruit yield by enhancing root activity and photosynthetic rates. - COLLAPSE
    February 2020
  • Research Article

    Characteristics of Domestic Net Type Melon in Hydroponic Spring Cultivars Using Coir Substrates
    Mi Young Lim, Su Hyun Choi, Ho Jeong Jeong, and Gyeong Lee Choi
    Hydroponics is considered to be useful for crop growth and quality control due to precision in nutrient supply and water management. This ... + READ MORE
    Hydroponics is considered to be useful for crop growth and quality control due to precision in nutrient supply and water management. This study investigated the adaptation of a hydroponic system on a coir substrate for spring season melon cultivars. Thirteen netted domestic cultivars grown in the spring season were evaluated in a Venlo glass greenhouse. Among the 13 cultivars, ‘Santafe’, ‘PMR Dalgona’, ‘Earl’s Miracle’, ‘Earl’s Crown’, and ‘Hero’ showed soluble solids content (SSC) of 12.0 ‑ 15.5°Brix, higher than that of ‘Earl’s Prugio’, ‘Earl’s Kingstar’, and ‘Fantasy’. At the harvest stage, plant length was longer in ‘Earl’s Kingstar’, ‘Earl’s Fantasy’, ‘Earl’s Crown’, ‘Santafe’, and ‘Earl’s Aibi’ than in the other cultivars, and leaf length was longer in ‘Earl’s Kingstar’, ‘Earl’s Prugio’, ‘Santafe’, ‘Earl’s Fantasy’, and ‘PMR Dalgona’. Fruit weight was highest in ‘Earl’s Kingstar’ (2.46 kg) and lowest in ‘Santafe’ (1.59 kg), while the other cultivars including ‘Earl’s Aibi’ had almost 2 kg weight. There was a negative correlation between fruit weight and sugar content. The results from this experiment may be useful for adaptability tests of domestic cultivars grown hydroponically using coir substrates. Ultimately, this study will be used as the basis for selecting a cultivar suitable for the export market, determining plant spacing, and establishing quality stabilization for melon hydroponics. - COLLAPSE
    February 2020
  • Research Article

    Comparison of Transport Methods and Wet Shipping Solutions for Cut Lily ‘Woori Tower’

    절화 백합 ‘Woori Tower’의 수송방식에 따른 품질 비교 및 효과적인 습식용액 규명

    Sang Im Oh and Ae Kyung Lee

    오상임, 이애경

    The aims of the present study were to compare the quality of cut flowers of the lily (Lilium longiflorum) ‘Woori Tower’ when ...

    본 연구는 수출용 절화 백합 ‘Woori Tower’의 건식수송과 습식수송에 따른 품질을 비교하고, 절화수명 연장 및 품질 향상을 위한 적정 습식용액을 구명하고자 ...

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    The aims of the present study were to compare the quality of cut flowers of the lily (Lilium longiflorum) ‘Woori Tower’ when exported via dry transport (DT) and wet transport and to identify a wet shipping solution that would improve both the vase life and quality of the cut flowers. Cut ‘Woori Tower’ flowers were treated with tap water (TW), Chrysal AVB (C. AVB, 1 mL·L-1), Chrysal SVB (C. SVB, 1 pill·3 L-1), 1-methylcyclopropene kit (1-MCP, 2 mg·L-1), GA4+7 + BA (100 mL· L-1), Vital Oxide (V.O, 2 mL·L-1), or H2 water (HW, 1.5 mg·L-1) as DT and wet shipping solutions. First and second flowers were in full bloom during the consumer stage (Day 7), with no significant differences between treatment groups. For the third flowers, only the C. SVB and GA4+7 + BA treatment groups were in full bloom, and neither the control group nor the TW group had bloomed at all. The vase life of the C. SVB treatment group and the GA4+7 + BA treatment group were 9.5 and 9.0 d, respectively, which were longer by 1.8 to 2.3 d, and tilting, petal abscission, and yellowing occurred slightly less in these groups than in the DT group. In particular, the C. SVB treatment was associated with a higher fresh weight change rate and a longer positive water balance (9 d), which were effective for quality maintenance. Fewer bacteria were detected in the C. SVB treatment group than in the DT, TW, or wet shipping solution treatment groups, and the ethylene production of the C. SVB group was lower, thereby improving vase life and freshness. Therefore, wet shipping was more effective than DT in improving the quality of cut lily flowers. In addition, C. SVB was the most effective among the tested wet shipping solutions in improving vase life and flower quality, likely by inhibiting ethylene production and improving water balance.


    본 연구는 수출용 절화 백합 ‘Woori Tower’의 건식수송과 습식수송에 따른 품질을 비교하고, 절화수명 연장 및 품질 향상을 위한 적정 습식용액을 구명하고자 수행하였다. 실험재료로는 longiflorum 계통의 절화 백합 ‘Woori Tower’(Lilium longiflorum ‘Woori Tower’)를 사용하였다. 처리는 건식(dry transport, DT)과 습식용액으로 수돗물(tap water, TW), Chrysal AVB(C. AVB) 1mL·L-1, Chrysal SVB(C. SVB) 1pill·3L-1, 1-MCP kit(1-MCP) 2mg·L-1, GA 4+7 + BA 100mL·L-1, Vital Oxide(V.O) 2mL·L-1, H2 water(HW) 1.5mg·L-1으로 하였다. 조사 결과, 소비자 단계(실험 7일)에서 1번화와 2번화는 모두 만개하였으나 처리 간의 유의한 차이가 없었다. 3번화의 경우 C. SVB 처리구와 GA4+7 + BA 처리구만 만개하였으며, 대조구와 TW 처리구에서 불개화 현상이 나타난 것으로 조사되었다. 절화수명 조사결과, C. SVB 처리구는 9.5일, GA4+7 + BA 처리구는 9.0일로 대조구보다 1.8 ‑ 2.3일 연장되었으며, 위조 및 꽃잎 탈리와 엽황화가 다소 적게 발생되었다. 특히, C. SVB 처리구는 생체중 변화율이 높게 유지되었고, 수분균형이 실험 9일에 부(-)의 값으로 떨어지며 품질 유지에 효과적이었다. C. SVB 처리구는 건식수송과 TW 처리구보다 박테리아가 적게 검출되었으며, 에틸렌 발생량 또한 적게 나타나 절화수명 및 선도유지에 효과적인 것으로 판단된다. 따라서, 습식수송이 건식수송에 비하여 절화 백합의 품질 향상에 효과적인 것으로 판단된다. 특히, 습식용액으로 C. SVB는 에틸렌 발생을 억제하고 높은 수분균형 유지를 통해 갈변과 엽황화 발생을 낮추어 절화수명 및 품질에 효과적인 것으로 판단된다.

    - COLLAPSE
    February 2020
  • Research Article

    Resistance of Commercial Radish Cultivars to Isolates of Fusarium oxysporum f. sp. raphani

    시들음병균 균주들에 대한 무 품종들의 저항성

    Soo Min Lee, Ji Hyun Lee, Kyoung Soo Jang, Yong Ho Choi, Hun Kim, and Gyung Ja Choi

    이수민, 이지현, 장경수, 최용호, 김 헌, 최경자

    Fusarium wilt of radish caused by Fusarium oxysporum f. sp. raphani (FOR) is one of the most severe diseases in radish cultivation ...

    무 시들음병은 Fusarium oxysporum f. sp. raphani (FOR)에 의해 발생하며, 무 재배지에서 심각한 피해를 주고 있는 병들 중 하나이다. 무 품종들의 ...

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    Fusarium wilt of radish caused by Fusarium oxysporum f. sp. raphani (FOR) is one of the most severe diseases in radish cultivation. To define resistance characteristics of radish cultivars to FOR, we tested virulence of 8 FOR isolates in a susceptible cultivar of radish, ‘Backchun’. Area under disease progress curve (AUDPC) of Fusarium wilt on the cultivar caused by FOR 60A, 59A, 57A, 147A, KR1, NW1, HN and JHW was 6.7, 14, 16, 27, 27, 30, 35 and 35, respectively. Thus, virulence of FOR HN and JHW was the strongest, followed by FOR NW1, KR1, 147A, 57A, 59A and 60A isolates. In addition, we investigated development of Fusarium wilt on 60 commercial radish cultivars caused by FOR KR1, a medium virulent isolate of them. From the results, 25 radish cultivars were selected for further study. When the cultivars were inoculated with the seven FOR isolates except for KR1, mean disease index of the cultivars by FOR 60A, 59A, 57A, 147A, NW1, HN, and JHW isolates were 0.5, 0.7, 1.2, 2.3, 2.8, 3.7 and 3.9, respectively. And the number of resistant radish cultivars to FOR 60A, 59A, 57A, 147A, NW1, HN, and JHW were 21, 20, 13, 7, 2, 1 and 1, respectively. Furthermore, development of Fusarium wilt in each cultivar was positively correlated with the virulence of FOR isolates. Taken together, these results suggest that resistance of radish to FOR isolates is likely affected by the virulence, but not resulted from race differentiation.


    무 시들음병은 Fusarium oxysporum f. sp. raphani (FOR)에 의해 발생하며, 무 재배지에서 심각한 피해를 주고 있는 병들 중 하나이다. 무 품종들의 시들음병 저항성 특징을 조사하기 위하여, 감수성 품종인 ‘백춘’에 FOR 8개 균주를 접종하고 시들음병 발생을 조사하였다. FOR 60A, 59A, 57A, 147A, KR1, NW1, HN, JHW에 의한 ‘백춘’에서의 병진전곡선하면적(AUDPC)은 각각 6.7, 14, 16, 27, 27, 30, 35, 35였다. 즉, FOR 균주들의 병원력은 HN와 JHW 균주가 가장 강하였고, 그 다음은 NW1, 147A, 57A, 59A, 60A 순이었다. 그리고 이들 균주 중 중간 정도의 병원력을 나타내는 FOR KR1 균주를 사용하여 시판 무 품종 60개의 시들음병 발생을 조사하였다. 이로부터 시들음병 저항성 특성 실험을 위하여 25개 품종을 선발하였다. FOR KR1 균주를 제외한 7개 균주에 의한 선발한 25개 품종의 시들음병 발생을 조사한 결과, 무 시들음병균 FOR 60A, 59A, 57A, 147A, NW1, HN, JHW에 의한 평균 발병도는 각각 0.5, 0.7, 1.2, 2.3, 2.8, 3.7, 3.9였으며, 저항성 품종의 수는 각각 21개, 20개, 13개, 7개, 2개, 1개 및 1개 이었다. 그리고 각 품종에서의 시들음병 발생은 FOR 균주들의 병원력에 비례하였다. 이상의 결과로 무의 시들음병 저항성은 F. oxysporum f. sp. raphani 균주들의 병원력 정도에 따라 영향을 받는 것이며, 병원균의 레이스(race) 분화 때문이 아닌 것으로 생각되었다.

    - COLLAPSE
    February 2020
  • Research Article

    Chimerism Evaluation of ‘Hongrou Huyou’, a Grafted Chimera between Citrus changshan-huyou and Citrus unshiu
    Min Zhang, Zequn Zhang, Qun Wu, Fuzhi Ke, Jianguo Xu, Siqing Zhao, Gang Wang, and Chi Zhang
    Chimeras occur spontaneously or artificially and are valuable in horticultural crop breeding. A new diploid citrus chimera, named ‘Hongrou Huyou’ (abbreviated HH ... + READ MORE
    Chimeras occur spontaneously or artificially and are valuable in horticultural crop breeding. A new diploid citrus chimera, named ‘Hongrou Huyou’ (abbreviated HH) (Citrus changshan-huyou + C. unshiu), was found during a bud sports investigation in China. The morphology, flesh carotenoid content, and molecular markers were evaluated in HH and the two grafted donors. The results showed that characteristics derived from L2/L3, such as the fruit size, winged leaf, seed, pollen, and rind aroma, were similar to those of C. changshan-huyou (CH), whereas the juice sac and stomatal characteristics originating from L1 were the same as those of the satsuma mandarin. High-performance liquid chromatography analysis of carotenes from the flesh of HH showed that the content was the same as that of the satsuma mandarin, with β-cryptoxanthin producing the main carotenoid spectrum, whereas lutein and violaxanthin were the main carotenoids in CH. Nuclear simple sequence repeat, chloroplast simple sequence repeat, and mitochondrial simple sequence repeat analyses showed that the leaves, outer pericarp (epidermis and flavedo), segment wall, and juice sac of HH contained the nuclear, chloroplast, and mitochondrial genomes of both donors; however, the albedo of HH only contained the genetic material of CH. Thus, HH is confirmed to be a periclinal chimera that consists of L1 from C. unshiu and L2/L3 from CH. - COLLAPSE
    February 2020