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2020 Vol.38, Issue 5 Preview Page

Research Article

Influences of Three Oidiodendron maius Isolates and Two Inorganic Nitrogen Sources on the Growth of Rhododendron kanehirae
Lei-Chen Lin1
,
Wan-Rou Lin2
,
Yu-Chen Hsu1
,
Hung-Yu Pan3*
1Department of Forestry and Natural Resources, National Chiayi University, College of Agriculture
2Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI)
3Department of Applied Mathematics, National Chiayi University, College of Science and Engineering
* Corresponding Author
31 October 2020. pp. 742-753
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Abstract

Rhododendron kanehirae Wilson is an endemic species of Taiwan that is listed as extinct in the wild by the Red List of Vascular Plants of Taiwan. R. kanehirae seedlings can form mycorrhizae with the ericoid mycobiont Oidiodendron maius. A completely randomized experimental design was conducted to investigate the effects of three different O. maius isolates (strains CBS110450, RhYM3, and RooDK1) as well as nitrate and ammonium (inorganic nitrogen) on the growth of R. kanehirae by evaluating height and shoot, root, and total fresh weight. Three isolates of O. maius showed different colony morphology and growth rate, which was further verified by molecular analysis. The results showed that application of ammonium fertilizer improved the height and shoot, root, and total fresh weight of all of the R. kanehirae seedlings. Among them, seedlings inoculated with RhYM3 had the highest fresh weights and heights. By applying an appropriate amount of nitrogen combined with RhYM3 inoculation, the rhizome of R. kanehirae grew well. The information provided here is crucial for the conservation of R. kanehirae, as this species is on the verge of extinction.

Keywords
ammonium
ericoid mycorrhiza
fresh weight
nitrate
shoot-root ratio

References
1
Argent G (2015) Rhododendron of subgenus Vireya, 2nd ed. Royal Botanic Garden Edinburgh in association with The Royal Horticultural Society, UK
2
Bradley R, Burt AJ, Read DJ (1981) Mycorrhizal infection and resistance to heavy metal toxicity in Calluna vulgaris. Nature 292:335-337. doi:10.1038/292335a0
10.1038/292335a0
3
Bradley R, Burt AJ, Read DJ (1982) The biology of mycorrhiza in the Ericaceae. VIII. The role of mycorrhizal infection in heavy metal resistance. New Phytol 91:197-209. doi:10.1111/j.1469-8137.1982.tb03306.x
10.1111/j.1469-8137.1982.tb03306.x
4
Bücking H, Kafle A (2015)Role of arbuscular mycorrhizal fungi in the nitrogen uptake of plants: current knowledge and research gaps. Agronomy 5:587-612. doi:10.3390/agronomy5040587
10.3390/agronomy5040587
5
Cairney JWG, Meharg AA (2003) Ericoid mycorrhiza: a partnership that exploits harsh edaphic conditions. Eur J Soil Sci 54:735-740. doi:10.1046/j.1351-0754.2003.0555.x
10.1046/j.1351-0754.2003.0555.x
6
Cairney JWG, Sawyer NA, Sharples JM, Meharg AA (2000)Intraspecific variation in nitrogen source utilization by isolates of the ericoid mycorrhizal fungus Hymenoscyphus Ericae (Read) Korf and Kernan. Soil Biol Biochem 32:1319-1322. doi:10.1016/S0038-0717(00)00025-0
10.1016/S0038-0717(00)00025-0
7
Chien CY (2016) A Study on effects of axenic synthesis and characteristics of root fungal endophytes isolated from Rhododendron hyperythrum Hay. in Yangmingshan area. Master Thesis, National Chiayi University, Taiwan
8
Dalpé Y (1986) Axenic synthesis of ericoid mycorrhiza in Vaccinium angustifolium Ait. by Oidiodendron species. New Phytol 103:391-396. doi:10.1111/j.1469-8137.1986.tb00624.x
10.1111/j.1469-8137.1986.tb00624.x
9
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13-15. doi:10.2307/2419362
10.2307/2419362
10
Editorial Committee of the Red List of Taiwan Plant (2017) The Red List of Vascular Plants of Taiwan, 2017. Endemic Species Research Institute, Forestry Bureau, Council of Agriculture, Executive Yuan and Taiwan Society of Plant Systematics. https://www.tesri.gov.tw/Uploads/userfile/A6_2/2019-02-25_1315069780.pdf Accessed 25 February 2019
11
Fayaz A (2012) Biodiversity of the Vireya group of Rhododendron L. (Ericaceae) collections in New Zealand and their potential contribution to international conservation(PhD). Turitea, New Zealand: Massey University. Retrieved 2017-09-26
12
Frank AB (1885) Über die auf Würzelsymbiose beruhende Ehrnährung gewisser Bäum durch unterirdische Pilze. Ber dt bot 3:128-145
13
Gerrits van den Ende AHGG, Hoog GSD (1999) Variability and molecular diagnostics of the neurotropic species Cladophialophora bantiana. Stud Mycol 43:151-162
14
Gibbs D, Chamberlain D, Argent G (2011) The red list of Rhododendrons. Botanic Gardens Conservation International, Richmond, UK
15
Gibson BR, Mitchell DT (2005) Influence of pH on copper and zinc sensitivity of ericoid mycobionts in vitro. Mycorrhiza 15:231-234. doi:10.1007/s00572-005-0356-3
10.1007/s00572-005-0356-315789234
16
Grossnickle SC (2005a) Seedling size and reforestation success. How big is big enough? In: Colombo, S.J., comp. Proceedings, the thin green line: a symposium on the state-of-the-art in reforestation. Forest Research Information Paper 160. Sault Saint Marie, ON, Canada: Ministry of Natural Resources, Ontario Forest Research Institute 144-149
17
Grossnickle SC (2005b) Importance of root growth in overcoming planting stress. New Forest 30:273-294. doi:10.1007/s11056-004-8303-2
10.1007/s11056-004-8303-2
18
Grossnickle SC (2012) Why seedlings survive: importance of plant attributes. New Forest 43:711-738. doi:10.1007/s11056-012-9336-6
10.1007/s11056-012-9336-6
19
Hall BG (2013)Building phylogenetic trees from molecular data with MEGA. Mol Biol Evol 30:1229-1235. doi:10.1093/molbev/mst012
10.1093/molbev/mst01223486614
20
Hall TA (2011) BioEdit: An important software for molecular biology. GERF Bull Biosci 2:60-61
21
Hambleton S, Currah RS (1997) Fungal endophytes from the roots of alpine and boreal Ericaceae. Can J Bot 75:1570-1581. doi:10.1139/b97-869
10.1139/b97-869
22
Harris RW (1992) Root-shoot ratios. J Arboric 18:39-42
23
Haynes RJ (1986) Uptake and assimilation of mineral nitrogen by plants. In RJ Haynes, KC Cameron, KM Goh, RR Sherlock eds, Mineral nitrogen in the plant-soil system. Academic Press, Orlando, FL, pp 303-378. doi:10.1016/B978-0-12-334910-1.50010-8
10.1016/B978-0-12-334910-1.50010-8
24
Ivetić V, Grossnickle S, Škorić M (2016) Forecasting the field performance of Austrian pine seedlings using morphological attributes. iForest 10:99-107. doi:10.3832/ifor1722-009
10.3832/ifor1722-009
25
Kirk PM, Cannon PF, David JC, Stalpers JA (2001) Ainsworth and Bisby's Dictionary of the Fungi (9th ed.). CAB International, Wallingford, UK
26
Li HL, Lu SY, Yang YP, Tseng YH (1998) Ericaceae. In Huang TC et al. eds, Flora of Taiwan, ED 2, Vol 4. Editorial Committee, Dept Bot, NTU, Taipei, Taiwan, pp 17-39
27
Lin LC, Chang TP, Chi TP, Hwang SY, Lui MH (2017) The compatibility of four Ericaceous plant root endophytic fungi with Rhododendron kanehirae. TW J of Biodivers 19:97-107
28
Lin LC, Lee MJ, Chen JL (2011) Decomposition of organic matter by the ericoid mycorrhizal endophytes of Formosan rhododendron (Rhododendron formosanum Hemsl.). Mycorrhiza 21:331-339. doi:10.1007/s00572-010-0342-2
10.1007/s00572-010-0342-220978913
29
Lin LC, Wang CS (2017) Influence of light intensity and photoperiod on the seed germination of four Rhododendron species in Taiwan. Pak J Biol Sci 20:253-259. doi:10.3923/pjbs.2017.253.259
10.3923/pjbs.2017.253.25929023037
30
Lin LC, Ye YS, Lin WR (2019) Characteristics of root-cultivable endophytic fungi fromRhododendron ovatum Planch. Braz J Microbiol 50:185-193. doi:10.1007/s42770-018-0011-8
10.1007/s42770-018-0011-830637639PMC6863216
31
MacKay MB, Gardiner SE (2017) Geographic analysis of Red List Rhododendron (Ericaceae) taxa by country of origin identifies priorities for ex situ conservation. Blumea 62:103-120. doi:10.3767/blumea.2017.62.02.05
10.3767/blumea.2017.62.02.05
32
Marschner H (2012) Marschner's mineral nutrition of higher plants, 3rd Ed. Academic Press, London, UK. doi:10.1016/C2009-0-63043-9
10.1016/C2009-0-63043-9
33
Martino E, Perotto S, Parsons R, Gadd GM (2003)Solubilization of insoluble inorganic zinc compounds by ericoid mycorrhizal fungi derived from heavy metal polluted sites. Soil Biol Biochem 35:133-141. doi:10.1016/S0038-0717(02)00247-X
10.1016/S0038-0717(02)00247-X
34
Michelsen A, Schmidt IK, Jonasson S, Quarmby C, Sleep D (1996) Leaf 15N abundance of subarctic plants provides field evidence that ericoid, ectomycorrhizal and non- and arbuscular mycorrhizal species access different sources of soil nitrogen. Oecologia 105:53-63. doi:10.1007/BF00328791
10.1007/BF0032879128307122
35
Miller AJ, Cramer MD (2005)Root nitrogen acquisition and assimilation. Plant Soil 274:1-36. doi:10.1007/s11104-004-0965-1
10.1007/s11104-004-0965-1
36
Piercey MM, Thormann MN, Currah RS (2002) Saprobic characteristics of three fungal taxa from ericalean roots and their association with the roots of Rhododendron groenlandicumand Picea marianain culture. Mycorrhiza 12:175-180. doi:10.1007/s00572-002-0166-9
10.1007/s00572-002-0166-912189471
37
Read DJ (1996)The structure and function of the ericoid mycorrhizal root. Ann Bot 77:365-374. doi:10.1006/anbo.1996.0044
10.1006/anbo.1996.0044
38
Rice AV, Currah RS (2001) Physiological and morphological variation in Oidiodendron maius. Mycotaxon 79:383-396
39
Schmid E, Oberwinkler F, Gomez LD (1995) Light and electron microscopy of a host-fungus interaction in the roots of some epiphytic ferns from Costa Rica. Can J Bot 73:991-996. doi:10.1139/b95-107
10.1139/b95-107
40
Stoyke G, Currah RS (1991) Endophytic fungi from the mycorrhizae of alpine ericoid plants. Can J Bot 69:347-352. doi:10.1139/b91-047
10.1139/b91-047
41
Thompson JD, GibsonTJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analyse tools. Nucleic Acids Res 25:4876-4882. doi:10.1093/nar/25.24.4876
10.1093/nar/25.24.48769396791PMC147148
42
Tseng YH (1993) Rhododendron kanehirae Wilson. Nature Conserv Quar 2:41-43. (In Chinese)
43
Upson R, Read DJ, Newsham KK (2007) Widespread association between the ericoid mycorrhizal fungus Rhizoscyphus ericae and a leafy liverwort in the maritime and sub-Antarctic. New Phytol 176:460-471. doi:10.1111/j.1469-8137.2007.02178.x
10.1111/j.1469-8137.2007.02178.x17888123
44
Usuki F, Narisawa K (2005) Formation of structures resembling ericoid mycorrhizas by the root entophytic fungus Heteroconium chaetospora within roots of Rhododendron obtusum var. Kaempferi. Mycorrhiza 15:61-64. doi:10.1007/s00572-004-0333-2
10.1007/s00572-004-0333-215517420
45
Vilgalys R, Hester M (1990)Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238-4246. doi:10.1128/jb.172.8.4238-4246.1990
10.1128/JB.172.8.4238-4246.19902376561PMC213247
46
Vohník M, Albrechtová J, Vosátka M (2005) The inoculation with Oidiodendron maius and Phialocephala fortinii alters phosphorus and nitrogen uptake, foliar C:N ratio and root biomass distribution in Rhododendron cv. Azurro. Symbiosis 40:87-96
47
Wang YZ, Lin LC (2014) A note on Oidiodendron maius in Taiwan. Collection and Research 27:1-3
48
Wei X, Chen J, Zhang C, Pan D (2016) A new Oidiodendron maius strain isolated from Rhododendron fortunei and its effects on nitrogen uptake and plant growth. Front Microbiol 7:1327. doi:10.3389/fmicb.2016.01327
10.3389/fmicb.2016.01327
49
Xiao G, Berch SM (1999) Organic nitrogen use by salal ericoid mycorrhizal fungi from northern Vancouver Island and impacts on growth in vitro of Gaultheria shallon. Mycorrhiza 9:145-149. doi:10.1007/s005720050299
10.1007/s005720050299
50
Zijlstra JD, Hof Pvan't, Baar J, Verkley GJM, Summerbell RC, Paradi I, Braakhekke WG, Berendse F (2005) Diversity of symbiotic root endophytes of the Helotiales in ericaceous plants and the grass, Deschampsia flexuosa. Stud Mycol 53:147-162. doi:10.3114/sim.53.1.147
10.3114/sim.53.1.147
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :5
  • Pages :742-753
  • Received Date : 2019-10-29
  • Revised Date : 2020-02-11
  • Accepted Date : 2020-07-21
  • DOI :https://doi.org/10.7235/HORT.20200067
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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