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2020 Vol.38, Issue 5 Preview Page

Research Article

Identification and Purity Test of Melon Cultivars and F1 Hybrids Using Fluidigm-based SNP Markers
D.S. Kishor1
,
Yoonji Noh1
,
Woon-Ho Song1
,
Gung Pyo Lee2
,
Jin-Kee Jung3
,
Eun-Jo Shim3
,
Sang-Min Chung1*
1Department of Life Sciences, College of Life Sciences & Biotechnology, Dongguk University
2Department of Plant Science and Technology, Chung-Ang University
3Seed Testing and Research Center, Korea Seed & Variety Service
* Corresponding Author
31 October 2020. pp. 686-694
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Abstract

In melon (Cucumis melo L.), the grow-out test (GOT) has been traditionally used as a genetic purity test. However, this method is time-consuming, space-demanding, and associated with ambiguous classification of the genotypes. Molecular markers have proved to be an efficient tool in genotyping analyses. In the present study, a total of 96 genome-wide single nucleotide polymorphism (SNP) markers differentiated 85 melon F1 hybrid plants from their parental lines and six other PT melon breeding lines via high-throughput Fluidigm genotyping. Of these, 39 SNP markers showed polymorphism between the parents. Additionally, SNP analysis and population structure analysis showed that several F1 hybrid plants were associated with outcrossing during the breeding program. Unweighted pair group method with arithmetic average (UPGMA) analysis revealed that most of the contaminated plants were closely sub-grouped with the 7_PT1 breeding line, suggesting possible outcrossing with 7_PT1. By combining with simple DNA extraction, the Fluidigm-based SNP marker analysis proved to be a simple and effective approach for the genetic purity analysis of F1 hybrids and melon cultivars.

Keywords
F1 hybrids and breeding lines
Fluidigm analysis
genetic purity analysis
melon
single nucleotide polymorphism (SNP)

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :5
  • Pages :686-694
  • Received Date : 2020-05-14
  • Revised Date : 2020-06-08
  • Accepted Date : 2020-06-27
  • DOI :https://doi.org/10.7235/HORT.20200062
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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